• 关键词：镰刀菌(Fusarium sp.)  2,4,6-三氯苯酚  生物降解  动力学  降解途径

• 基金项目：浙江省自然科学基金项目（No.LY16E080001）

• 蔡伟建
• 浙江工商大学食品与生物工程学院, 杭州 310018

• 潘俊雅
• 浙江工商大学环境科学与工程学院, 杭州 310018

• 李济吾
• 浙江工商大学环境科学与工程学院, 杭州 310018

• 摘要：为了解镰刀菌（Fusarium sp.）降解2，4，6-三氯苯酚（TCP）的因素影响规律，研究了温度、pH、外加碳源、氮源、氯离子及TCP浓度对其降解特性的影响，分析了其降解动力学与降解途径. 结论：镰刀菌能以TCP为唯一碳源和能源物质进行生长繁殖，TCP降解最适条件为：氮源NaNO₃（0.2 g·L^-1），30℃，pH=6～7. 外加碳源葡萄糖对降解TCP具有明显的抑制作用. 氯离子浓度低于0.2 g·L^-1时对降解TCP具有一定的促进作用，但随着氯离子浓度的增加，TCP的降解受到了抑制. 镰刀菌对TCP降解速率随着其浓度的升高而减缓. 镰刀菌能降解10～50 mg·L^-1的TCP，其降解反应符合零级降解动力学方程. 镰刀菌降解TCP过程中检测到2，6-二氯苯酚（RT 12.521 min），可推测TCP是通过2，6-二氯苯酚途径进行降解的.

• Abstract：The effects of temperature, pH, carbon and nitrogen sources, and concentrations of chloride ion and 2, 4, 6-trichlorophenol (TCP) on degradation of TCP by Fusarium sp. were investigated. The kinetic and pathway of TCP degradation using Fusarium sp. were discussed by UV scanning and GC-MS analysis. The results show that Fusarium sp. has a high capacity in degrading TCP in solution by using TCP as the sole carbon source and energy source to grow. The optimum values of pH and temperature for TCP degradation by Fusarium sp. are pH 6 and 30℃, respectively. The optimum nitrogen source is 0.2 g·L^-1 NaNO₃. Added glucose carbon source may restrain the degradation capacity. The degradation rates increase with increasing of concentration of chloride ion, but turn to decrease when the concentration of chloride ion is larger than 0.2 g·L^-1. The degradation rates also decrease with increasing of concentration of TCP. The degradation kinetics of TCP well accord with the zero order equation. TCP may be degraded via 2, 6-dichlorophenol pathway by Fusarium sp..

• Key words：Fusarium sp.  2, 4, 6-trichlorophenol  biological degradation  kinetics  degradation pathway

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