研究报告
张海涛,刘文斌,杨海君,张宇.一株耐盐高效苯酚降解菌的筛选、鉴定、响应面法优化与降酚动力学研究[J].环境科学学报,2016,36(9):3200-3207
一株耐盐高效苯酚降解菌的筛选、鉴定、响应面法优化与降酚动力学研究
- Isolation, identification, optimization via response surface methodology of a highly-efficient phenol-degrading salt-tolerant strain and its degradation kinetics
- 基金项目：湖南省自然科学基金项目（No.2015JJ2079）；湖南省研究生科研创新基金课题（No.CX2015B290）
- 作者
- 单位
- 张海涛
- 湖南农业大学植物保护学院, 长沙 410128
- 刘文斌
- 湖南农业大学植物保护学院, 长沙 410128
- 杨海君
- 湖南农业大学植物保护学院, 长沙 410128
- 张宇
- 湖南农业大学植物保护学院, 长沙 410128
- 摘要：从海利（常德）有限公司污水处理池水样中分离得到1株耐盐高效苯酚降解菌，该菌可在盐度1%～8%、pH4.0～10.0、温度15～40℃条件下以苯酚为唯一碳源生长，命名为zhtI.通过形态特征、生理生化反应、16S rDNA鉴定和BLAST序列比对构建系统发育树，确定菌株zhtI为一株不动杆菌Acinetobacter guillouiae；利用Plackett-Burman实验确定葡萄糖浓度、苯酚浓度、pH值为影响菌株zhtI降解苯酚的主要因素；采用中心组合实验设计，结合Box-Behnken实验设计及响应面法分析，确定菌株zhtI在苯酚浓度801.6 mg·L-1 、葡萄糖浓度5.56%、pH值7.3、接种量6%、温度30℃、盐度3%条件下培养72 h，菌株zhtI对苯酚的降解率达到93.23%.经降解动力学研究发现菌株zhtI对苯酚的最大耐受浓度为1700 mg·L-1，菌株zhtI降解苯酚的动力学模型符合典型的底物抑制模型，降酚动力学参数为：μmax=2.142 h-1，KS=126.952 mg·L-1，Ki=476.191 mg·L-1.
- Abstract：A highly-efficient phenol-degrading salt-tolerant strain zhtI was isolated from the sewage in Haley Ltd (Changde). The strain can survive using phenol as the sole carbon source (phenol used) under the condition of salinity 1%~8%, pH4.0~10.0, temperature 15~40℃. Based on the morphological, physiology characterization, biochemical reaction, phylogenetic analsysis through16S rDNA gene sequence and BLAST sequence alignment, zhtI was identified as Acinetobacter guillouiae. Glucose concentration, phenol concentration, pH were identified as three main factors affecting the biodegradation of phenol analyzed by Plackett-Burman experimental. Utilizing the central composite experimental design, together with the Box-Behnken experimental design and response surface method, it is confirmed that the phenol degradation rate reached 93.23% at phenol concentration 801.6 mg·L-1, glucose concentration 5.56%, pH7.3, inoculum 6%, temperature 30℃, salinity 3%, cultured 72 h. Through kinetics study, it is concluded that the strain zhtI maximum tolerated concentration of phenol was 1700 mg·L-1, the strain zhtI degradation of phenol kinetic model was in line with the typical substrate inhibition model, and its kinetic parameters were:μmax= 2.142 h-1, KS= 126.952 mg·L-1, Ki=476.191 mg·L-1.
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