• 关键词：Enterobacter sp.  孔雀石绿  脱色特性  代谢产物  代谢机理

• 基金项目：浙江省自然科学基金（No.LQ13C010001）；温州市水体污染控制与治理科技创新项目（No.S20140027）

• 都林娜
• 温州科技职业学院, 温州 325006

• 李刚
• 温州科技职业学院, 温州 325006

• 杨玉义
• 中国科学院武汉植物园, 武汉 430074

• 许方程
• 温州科技职业学院, 温州 325006

• 王阳
• 温州科技职业学院, 温州 325006

• 泮琇
• 温州科技职业学院, 温州 325006

• 摘要：采用单因素实验研究了各种操作因素对菌株Enterobacter sp. CV-v降解孔雀石绿的影响.结果表明，在供试碳源中，葡萄糖对脱色的促进效果最为显著，而供试氮源中，酵母粉对脱色的促进效果最优；同时，供试金属离子中，锰离子对脱色的促进效果最优.在pH=3.0～10.0、温度20～50℃之间时，菌株CV-v对孔雀石绿的12 h脱色率在90%以上.此外，该菌株可在6 h内完全脱色浓度低于500 mg·L^-1的孔雀石绿.动力学实验结果表明，该菌株对孔雀石绿脱色的动力学数据与一级动力学模型拟合度最好（R²=0.9755）.酶分析实验结果表明，锰过氧化物酶和孔雀石绿还原酶可能与菌株CV-v降解孔雀石绿相关.此外，代谢产物分析实验结果表明，菌株CV-v降解孔雀石绿的主要产物为二甲氨基二苯甲酮和4-羟基-N，N-二甲基苯铵.

• Abstract：Single factor experiments were employed to study the effect of various operational factors on the degradation of Malachite Green (MG) by Enterobacter sp. CV-v. Results indicate that among the tested carbon,nitrogen and metal ion sources,glucose,yeast extracts and Mn²⁺ showed the most significant enhancement effect for decolorization,respectively. After 12 h of incubation,over 90% of MG decolorization efficiency by strain CV-v was obtained under the conditions of pH 3.0~10,20~50℃. Moreover,this strain could completely decolorize <500 mg·L^-1 of MG in 6 h. Results of the kinetics study indicate that the data could be well fitted by the one-order kinetic model (R²=0.9755). Results of enzyme analysis show that manganese peroxidase and MG reductase might be related to the degradation of MG by strain CV-v. Besides,(dimethyl amino-phenyl)-phenyl-methanone and 4-hydroxyl-N,N-dimethylaniline were the main metabolic products of MG degradation by strain CV-v.

• Key words：Enterobacter sp.  Malachite Green  characteristics of decolorization  metabolic products  metabolic mechanism

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