• 关键词：纳米氧化锌  强化生物除磷  微生物代谢  群落结构变迁  可恢复性

• 基金项目：国家自然科学基金（No.21276236）；浙江工商大学研究生科技创新项目（No.3100XJ1514152）

• 鲁轩余
• 浙江工商大学环境科学与工程学院, 杭州 310012

• 胡哲太
• 浙江工商大学环境科学与工程学院, 杭州 310012

• 孙培德
• 浙江工商大学环境科学与工程学院, 杭州 310012

• 韩竞一
• 浙江工商大学环境科学与工程学院, 杭州 310012

• 楼成珂
• 浙江工商大学环境科学与工程学院, 杭州 310012

• 摘要：研究了纳米氧化锌（ZnO NPs）对强化生物除磷（EBPR）系统的长期作用机制，系统分析了EBPR系统在ZnO NPs长期抑制下宏观运行性能与微观结构的变化.结果发现，当进水开始添加ZnO NPs后，系统沉降性能随之降低.厌氧释磷速率与好氧吸磷速率均出现显著降低并降至0 mg·g^-1·h^-1（以每g MLSS释（吸）P量（mg）计，下同），从而使得系统丧失除磷效果.同时，厌氧段COD开始积累.ZnO NPs浓度增加至10 mg·L^-1时，多糖与蛋白质含量在抑制条件下均开始减少.通过高通量技术对微观层面进行分析发现，ZnO NPs将会严重抑制聚磷菌的正常生长.ZnO NPs对系统内不同细菌有截然不同的作用，Proteobacteria门在试验过程中比例减小，而Bacteroidetes门却受到促进作用.恢复阶段，较低浓度（2 mg·L^-1）抑制条件下，EBPR系统恢复速度与程度均优于高浓度（6、10 mg·L^-1）抑制条件.然而，即使系统得到一定程度恢复也难以恢复到初始水平.

• Abstract：The long-term effect of zinc oxide nanoparticles (ZnO NPs) on enhanced biological phosphorus removal (EBPR) was studied. Operational performance and microbial community structure were analyzed in the inhibitory experiment. Settleability declined when ZnO NPs were added. Phosphorus release and uptake were also seriously affected by ZnO NPs loading. COD could not be removed in the anaerobic stage. Synthesis and consumption of EPS were severely inhibited when ZnO NPs concentration was 10 mg·L^-1. High throughput sequencing technology was applied to analyze microbial community structure. Results suggested that the growth of PAOs was depressed in the system. The toxicity of ZnO NPs was dependent on the species of bacteria. Proteobacteria decreased in the test, while Bacteroidetes presented an opposite trend. In the recovery stage, EBPR showed better reversibility in lower concentration of ZnO NPs (2 mg·L^-1). Moreover, the system was also difficult to return to the initial level in the recovery stage.

• Key words：Zinc oxide nanoparticles  enhanced biological phosphorus removal  microbial metabolism  microbial community variation  recovery

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