• 关键词：厌氧发酵  餐厨垃圾  油酸钠  脂肪酸  氢分压  微生物计数

• 基金项目：公益性行业（农业）科研专项经费（No.201303099-01）

• 王星
• 农业部沼气科学研究所, 成都 610041

• 尹小波
• 1. 农业部沼气科学研究所, 成都 610041;2. 农业部农村可再生能源开发利用重点实验室, 成都 610041

• 张敏
• 1. 农业部沼气科学研究所, 成都 610041;2. 农业部农村可再生能源开发利用重点实验室, 成都 610041

• 李强
• 1. 农业部沼气科学研究所, 成都 610041;2. 农业部农村可再生能源开发利用重点实验室, 成都 610041

• 周正
• 1. 农业部沼气科学研究所, 成都 610041;2. 农业部农村可再生能源开发利用重点实验室, 成都 610041

• 贺静
• 1. 农业部沼气科学研究所, 成都 610041;2. 农业部农村可再生能源开发利用重点实验室, 成都 610041

• 耿宇聪
• 1. 农业部沼气科学研究所, 成都 610041;2. 农业部农村可再生能源开发利用重点实验室, 成都 610041

• 李政伟
• 农业部沼气科学研究所, 成都 610041

• 摘要：为了研究餐厨垃圾油脂中含有的长链脂肪酸（主要是油酸）对两相厌氧发酵中利用乙酸产甲烷过程的影响，采用中温（35±1）℃批式发酵，以浓度为10 g·L^-1的餐厨垃圾厌氧发酵产甲烷相出水为接种液，以乙酸为底物，考察了添加不同浓度油酸钠对累积甲烷产量、产甲烷古菌数量以及发酵过程中乙酸代谢和氢气分压的影响.结果表明：①产甲烷延滞期随油酸钠浓度增加而延长.②油酸钠浓度低于6.0 g·L^-1的处理组经过延滞期后乙酸代谢恢复正常，可以完全被转化为甲烷，且部分油酸钠也可被转化为甲烷；油酸钠浓度高于8.0 g·L^-1的处理组乙酸代谢受到严重抑制，只有少量甲烷生成；油酸钠生成中间代谢产物，且甲烷转化率为0.③油酸钠对厌氧发酵中产甲烷古菌有严重的抑制作用，产甲烷古菌数量大幅度减少是乙酸代谢过程受到抑制的主要原因.④油酸钠的添加量超过6.0 g·L^-1时，在发酵初期会引起产甲烷相中氢分压的升高，也证实了油酸钠对厌氧发酵的抑制作用.

• Abstract：In order to study the effect of long-chain fatty acid, which was mainly present as oleate in food waste oil, on the process of acetate metabolism in two-phase anaerobic digestion of food waste, batch fermentation experiments were conducted at 35℃ to investigate the variation of cumulative methane yields, methanogens abundance and the effect on acetate and H₂ metabolism with different sodium oleate added, which used food waste anaerobic digestion effluent (10 g·L^-1) as inoculum and acetate as substrate respectively. The results indicate: ① the lag phase of anaerobic digestion was prolonged with the increasing concentration of sodium oleate, ② when the load of sodium oleate was lower than 6.0 g·L^-1, the acetate metabolism returned to normal and part of sodium oleate was converted to methane after lag phase, whereas, the acetate metabolism was inhibited severely and sodium oleate conversion was completely inhibited when the load of sodium oleate were higher than 8.0 g·L^-1, ③ sodium oleate had significant adverse effect on methanogens and was main reason for inhibiting acetate metabolism that the quantity of methanogens sharply decreased, ④ sodium oleate with concentration beyond 6.0 g·L^-1 would result in the raise of hydrogen partial pressure in the initial stage of experiment, which also indicated the inhibition effect of sodium oleate again.

• Key words：anaerobic digestion  food waste  fatty acids  (VFA)  sodium oleate  hydrogen partial pressure  microorganism count

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