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研究报告

Hepatic and renal tissue oxidative damage induced by dermal exposure to diisononyl phthalate in Balb/c mice

摘要：作为“绿色换代产品”，邻苯二甲酸二异壬酯（DINP）在全球范围逐渐取代邻苯二甲酸二（2-乙基己）酯（DEHP）成为聚氯乙烯制品的主要增塑剂.但人体DINP暴露也可能存在健康隐患，故近年来受到越来越多环境科学领域专家的关注.为了探讨DINP皮肤暴露是否对机体有氧化损伤的作用，本研究以Balb/c小鼠为模式生物进行实验，42只雄性Balb/c小鼠随机被分为5个DINP染毒组（0.02、0.2、2、20、200 mg·kg^-1）和1个溶剂对照组，每组7只.皮肤染毒28 d后，用所获得的肝和肾组织匀浆测定活性氧自由基（ROS）、丙二醛（MDA）、还原型谷胱甘肽（GSH），用所获得的肝和肾细胞测定DNA-蛋白质交联（DPC）系数，同时观察小鼠背部皮肤的形态变化情况.实验结果表明，随着DINP染毒剂量的升高，脏器系数、ROS和MDA含量及DPC系数逐渐上升，GSH含量逐渐降低，各指标均呈现出一定的剂量-反应关系（p<0.05，p<0.01）.研究表明，较高剂量的DINP（≥20 mg·kg^-1）亦可通过皮肤暴露的方式造成小鼠肝脏和肾脏组织细胞的氧化损伤.

Abstract：As a "green generation product", di-isononyl phthalate (DINP) has gradually replaced di (2-ethylhexyl) phthalate (DEHP) as the major plasticizer for polyvinyl chloride (PVC) products in the world. However, DINP has also received considerable attention because of their high potential for human exposure and toxicity. In this study, BALB/c mice were employed as an animal model to explore the oxidative damage induced by DINP via dermal exposure. 42 male Balb/c mice were divided into six groups and five DINP exposure groups were exposed to DINP with different doses (0.02, 0.2, 2, 20, 200 mg·kg^-1) while the control used saline solution. After 28 consecutive days, reactive oxygen species (ROS), reduced glutathione (GSH) and malondialdehyde (MDA) obtained by hepatic and renal tissue homogenates were measured. DNA-protein crosslinks (DPC) coefficient in liver and kidney cells was derived. The skin lesions of the back of mice were also examined. Experimental results showed that the levels of organ coefficients, ROS, MDA, and DPC coefficients increased gradually in a dose-dependent manner, whereas GSH content decreased accordingly. These experimental results suggested that DINP at higher doses (≥20 mg·kg^-1) can induce the oxidative stress via dermal exposure in mice liver and kidney.