• 关键词：1-氨基蒽醌-2-磺酸  嗜吡啶红球菌GF3  降解

• 基金项目：哈尔滨工业大学城市水资源与水环境国家重点实验室开放基金项目（No.QA201412）

• 臧苗苗
• 大连理工大学环境学院工业生态与环境工程教育部重点实验室, 大连 116024

• 吕红
• 大连理工大学环境学院工业生态与环境工程教育部重点实验室, 大连 116024

• 周集体
• 大连理工大学环境学院工业生态与环境工程教育部重点实验室, 大连 116024

• 管玉江
• 台州学院生命科学学院, 台州 317000

• 王竞
• 大连理工大学环境学院工业生态与环境工程教育部重点实验室, 大连 116024

• 关小凡
• 大连理工大学环境学院工业生态与环境工程教育部重点实验室, 大连 116024

• 郭婉茜
• 哈尔滨工业大学城市水资源与水环境国家重点实验室, 哈尔滨 150090

• 摘要：从受溴氨酸污染的泥土中分离出1株蒽醌染料中间体1-氨基蒽醌-2-磺酸（简称ASA-2）降解菌株GF3.经形态学观察和16S rDNA序列分析，鉴定该菌株为嗜吡啶红球菌.研究了该菌对ASA-2脱色的特性，并利用液相-质谱联用仪初步分析了ASA-2降解终产物.研究结果表明，外加蛋白胨、酵母膏和水解酪蛋白均能促进ASA-2的生物脱色，其中蛋白胨促进作用最为明显.进一步的研究发现多种氨基酸可加速ASA-2的生物脱色过程，其中L-亮氨酸促进效果最好.ASA-2脱色的最适环境条件为pH 8.0、30℃和150 r·min^-1.最适条件下，菌株GF3可使108 mg·L^-1的ASA-2在30 h内脱色率达95%以上，TOC去除率为62%.紫外-可见波谱显示，当ASA-2水溶液由红色褪成无色时，ASA-2的特征吸收峰完全消失，并在340 nm产生了新峰.进一步分析发现，ASA-2降解终产物质荷比为260，初步推测产物为3-氨基-4-磺酸基邻苯二甲酸.此外，菌株GF3还可以降解溴氨酸、蒽醌-2-磺酸钠和蒽醌-2-羧酸.

• Abstract：An anthraquinone dye intermediate 1-amino-anthraquinone-2-sulfonic acid (ASA-2)-degrading strain GF3 was isolated from the bromamine acid-contaminated soil samples. Based on morphological observation and 16S rDNA sequence analysis, strain GF3 was identified as Rhodococcus pyridinivorans. The decolorization of ASA-2 by strain GF3 was studied and ASA-2 degradation product was analyzed using high performance liquid chromatography-mass spectrometry (HPLC-MS). The results show that additional peptone, yeast extract and casamino acid could promote ASA-2 bio-decolorization, among which the accelerating effect of peptone was the most obvious. Further experimental results demonstrate that many amino acids could accelerate the process of ASA-2 bio-decolorization, and L-leucine had the most significantly accelerating effect on ASA-2 decolorization and cell growth. The optimal environmental conditions for ASA-2 bio-decolorization were pH 8.0, 30℃ and 150 r·min^-1. Under the optimal conditions, the decolorization efficiency of 108 mg·L^-1 ASA-2 could reach over 95% in 30 h, and TOC removal rate was 62%. In UV-Visible spectra, the characteristic absorption peaks of ASA-2 totally disappeared and a new peak at 340 nm appeared when the red color of ASA-2 aqueous solution faded to be colorless. Further analysis found that the mass to charge ratio of the end product from ASA-2 degradation was 260. Thus, the degradation product was proposed to be 3-amino-4-sulfophthalic acid. In addition, strain GF3 could also degrade bromamine acid, anthraquinone-2-sulfonate and anthraquinone-2-carboxylic acid.

• Key words：1-amino-anthraquinone-2-sulfonic acid  Rhodococcus pyridinivorans GF3  degradation

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