• 关键词：石油降解  赤红球菌  GC-MS  含油废水

• 基金项目：国家自然科学基金（No.31272694）

• 刘猛
• 兰州理工大学石油化工学院, 兰州 730050

• 陈吉祥
• 兰州理工大学石油化工学院, 兰州 730050

• 周永涛
• 中石油北京天然气管道有限公司, 北京 100101

• 赵霞
• 兰州理工大学石油化工学院, 兰州 730050

• 孔令姣
• 兰州理工大学石油化工学院, 兰州 730050

• 刘元利
• 兰州理工大学石油化工学院, 兰州 730050

• 周敏
• 兰州理工大学石油化工学院, 兰州 730050

• 摘要：从石油污染土壤筛选出一株高效石油降解菌JC-106，经细菌形态学、生理生化及16S rDNA 序列分析鉴定为赤红球菌（Rhodococcus rubber）.在温度 15～40℃、初始pH 6～8、盐度0～4%条件下培养生长良好.该菌能利用十二烷、二十四烷、正辛烷、邻苯二酚、蒽、萘为唯一碳源生长.在较低温度下能有效降解原油，在 15和 35℃培养 15 d 对原油降解率分别为 41.61% 和 58.18%，GC-MS分析发现原油组分中正构烷烃（C₁₄～C₄₄）降解率达到 96.13%.在含1000 mg·L^-1原油的人工废水中加入2%（V/V）赤红球菌JC-106菌悬液，采用SBR间歇式活性污泥法处理含油废水，15 d后出水COD、NH₄⁺-N、TP平均去除率分别为96.49%、96.88%、99.15%，原油去除率为92.43%，对照组原油去除率仅为53.80%.JC-106 在含油废水中稳定生长，数量维持在4.8×10¹⁰～1.0×10¹¹ cfu·mL^-1 左右.

• Abstract：An oil-degrading bacterium JC-106 was isolated from the oil contaminated soil. The strain was preliminarily identified as Rhodococcus rubber by morphological, physiological biochemical characteristics and 16S rDNA sequence analysis. The strain JC-106 grew well under the conditions of temperature ranging from 15 to 40℃, the initial pH ranging from 6 to 8 and the salinity ranging from 0 to 4%. It grew well in broth containing different organic substrates as sole carbon source, such as dodecane, tetracosane, n-octane, catechol, anthracene and naphthalene. The strain could efficiently degrade crude oil at low temperature. The degradation rates for crude oil were 41.61% and 58.18% when it was cultured in minimal medium with crude oil as sole carbon source at 15 and 35℃ for 15 days. Most of the n-alkanes (C₁₄~C₄₄) in crude oil were degraded and the total degradation rate reached up to 96.13% by GC-MS analysis. In the case of oily wastewater treatment by Rhodococcus rubber JC-106 using sequencing batch reactor activated sludge process, the average removal rates of COD, NH₄⁺-N, TP were respectively measured to be 96.49%、96.88% and 99.15% by adding of 2% (V/V) of Rhodococcus rubber JC-106 cell solution to the artificial wastewater containing 1000 mg·L^-1 of crude oil for 15 days. The oil removal rate was measured to be as high as 92.43% in the experimental group containing the oil-degrading bacterium JC-106,compared to 53.80% in the control group. The bacterium grew well in oily wastewater with a titer of 4.8×10¹⁰~1.0×10¹¹cfu·mL^-1.

• Key words：oil-degrading  Rhodococcus rubber  GC-MS  oily wastewater

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