• 关键词：混合菌  去除率  固定化  响应面法  Cr³⁺  辛基酚聚氧乙烯醚  单因素

• 基金项目：湖南省自然科学基金项目（No.2015JJ2079）；湖南省研究生科研创新基金课题（No.CX2015B290）

• 张海涛
• 湖南农业大学植物保护学院, 长沙 410128

• 杨海君
• 湖南农业大学植物保护学院, 长沙 410128

• 彭晓霞
• 湖南农业大学植物保护学院, 长沙 410128

• 刘文斌
• 湖南农业大学植物保护学院, 长沙 410128

• 摘要：以微紫青霉菌B5和醋酸钙不动杆H1按照1：1配置成混合菌Z1，并分别以聚乙二醇、改性秸秆、海藻酸钠对混合菌Z1进行包埋，探究其对Cr³⁺和OPnEO的同步去除处理情况，结果得到OPnEO的去除率分别为53.42%、46.29%、55.14%；而Cr³⁺的去除率分别达到58.41%、54.24%、60.95%，确定海藻酸钠为Z1的最佳包埋材料.以Cr³⁺去除率为响应值，利用Plackett-Burman实验确定Cr³⁺的初始浓度、pH值、温度为影响混合菌Z1去除Cr³⁺的3个主要因素；运用最陡爬坡实验设计，结合Box-Behnken实验设计及响应面法分析，确定海藻酸钠固定化混合菌Z1在初始pH 7.64、Cr³⁺初始浓度856.34 mg·L^-1、温度30.39℃、OPnEO初始浓度950 mg·L^-1、外加2 mL胰蛋白胨条件下培养7 d，Cr³⁺的去除率达到77.69%，比单因素实验条件下Cr³⁺的去除率提高7%.

• Abstract：The culture Z1,which was mixed with Penicillium janthinellum B5 and Acinetobacter calcoaceticus H1 with the proportion of 1:1, was imbedded with polyethylene glycols, modified straw and alginate, respectively, for simultanueous removal of Cr³⁺ and OPnEO. The respective removal rates of OPnEO were 53.92%, 46.99% and 55.14%, and the removal rates of Cr³⁺ were 58.41%, 54.24% and 60.95%. Alginate was confirmed as the optimum embedding material. In the Plackett-Burman experiment, the initial Cr³⁺ concentration, pH, temperature, were ascertained as three influencing factors for Cr³⁺ removal by the mixed culture Z1. The steepest ascent experimental design, Box-Behnken experimental design and response surface method analysis have confirmed that the removal rate of Cr³⁺ reached 77.69% under the optimal condition, which was initial pH 7.64, initial Cr³⁺ concentration at 856.34 mg·L^-1, temperature 30.39℃, initial OPnEO concentration at 950 mg·L^-1, 2 mL tryptone and 7 d of cultivation, the removal rate of Cr³⁺ significantly increased 7% than those under the experimental conditions of single factor.

• Key words：mixed bacteria flora  removal rate  immobilization  response surface method  Cr³⁺  octylphenol ethoxylates  single factor

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