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研究报告

杨金焕,宗磊,贡晨雪,卢德赵,许颖龄,胡潇,王萃.环境浓度水平的苯并[b]荧蒽对巨噬细胞RAW264.7的作用[J].环境科学学报,2017,37(3):1133-1138

Effects of benzo [b] fluoranthene on RAW264.7 cells at environmental concentration level

摘要：为了研究环境浓度下多环芳烃苯并[b]荧蒽对巨噬细胞RAW264.7的损伤情况，我们用MTT法检测苯并[b]荧蒽对RAW264.7细胞增殖活力的影响，用试剂盒检测乳酸脱氢酶（LDH）及炎症因子的释放量、细胞内SOD酶活力、脂质过氧化产物MDA含量及活性氧自由基ROS水平，用FITC标记的大肠杆菌（E.coli）检测苯并[b]荧蒽对巨噬细胞吞噬能力的影响.结果显示环境浓度（10~80 nmol·L^-1）下的苯并[b]荧蒽能够抑制RAW264.7细胞的增殖活力，增加乳酸脱氢酶LDH的释放量.诱导活性氧自由基的形成，提高超氧化物歧化酶（SOD）活力及脂质过氧化产物MDA的含量.增加炎症因子IL-6，IL-1β和TNF-a的分泌，降低吞噬能力.该研究结果表明，苯并[b]荧蒽对巨噬细胞RAW264.7具有一定的毒性作用；能够引起RAW264.7细胞产生氧化应激；激活炎症反应；降低巨噬细胞的吞噬能力.

Abstract：This study aimed to investigate the adverse effects of benzo[b]fluoranthene on RAW264.7 cells at environmental concentration level. Cytotoxicity caused by benzo[b]fluoranthene was determined by MTT assay. The SOD activity and the MDA content of the RAW264.7 cells were measured according to the manufacturer's instructions. The secreted cytokines including tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) and interleukin-1β (IL-1β) were determined by Enzyme-Linked Immunosorbent Assay. Phagocytic ability was measured through the co-incubation with the configured Escherichia Coli (K-12 strain) Bio Particles^®.fluorescein conjugate. Our results show that benzo[b] fluoranthene reduced the proliferation of RAW264.7 cells, induced the production of the reactive oxygen species (ROS), increased the activity of SOD, LDH and the content of MDA, enhanced the levels of inflammatory cytokine, and finally impaired the phagocytic ability of RAW264.7 cells at concentrations of 10 nmol·L^-1 to 80 nmol·L^-1. The study suggests that benzo[b] fluoranthene can produce cytotoxicity, induce the oxidative stress and inflammatory response and weaken the phagocytic ability of RAW264.7 cells.