• 关键词：渭河  微生物多样性  16S rDNA  T-rflp技术  高通量测序

• 基金项目：教育部高等学校博士学科点专项科研基金（No.20136118120022）；陕西省科技统筹创新工程计划项目（No.2013SZS02-P01）

• 刘睿
• 西安理工大学西北旱区生态水利工程国家重点实验室, 西安 710048

• 吴巍
• 西安理工大学西北旱区生态水利工程国家重点实验室, 西安 710048

• 周孝德
• 西安理工大学西北旱区生态水利工程国家重点实验室, 西安 710048

• 岳泽滢
• 中国建筑西北设计研究院有限公司, 西安 710018

• 赵平歌
• 1. 西安理工大学西北旱区生态水利工程国家重点实验室, 西安 710048;3. 西安工业大学建筑工程学院, 西安 710021

• 摘要：基于T-rflp技术和高通量测序技术，分析了渭河在平水期、枯水期和丰水期的浮游细菌群落变化特征，并采用冗余分析和典范对应分析识别了不同水文时期影响细菌群落的关键环境因子.结果表明，渭河水体浮游细菌群落空间和季节差异明显，且季节性差异比空间差异更加显著.平水期、枯水期和丰水期渭河（陕西段）干流浮游细菌群落Shannon多样性指数分别在2.13~2.82、2.05~2.84和2.61~2.91之间.平水期浮游细菌多样性指数空间差异最大（RSD=16.75%），样点间群落结构相似度最低（26.8%），流域优势T-RF片段数最多（23种）；丰水期浮游细菌Shannon指数空间差异最小（RSD=9.27%），样点间群落相似度最高（62.6%），且检出的优势片段数最少（12种）.咸阳-西安段是浮游细菌群落多样性最低、优势菌群结构最单一的河段.河流水体细菌群落在不同时期的关键环境驱动因子不同，而其中悬浮颗粒物（TSS）浓度是不同水文时期都不可忽视的关键影响因子.高通量分析结果表明，丰水期渭河水体浮游细菌物种涉及21个已知细菌门类和26个候选门类，其中，变形菌门（Proteobacteria）和拟杆菌门（Bacteroidetes）共同的相对丰度占比达到75%以上，是最主要的细菌类群.汛期渭河干流各样点浮游细菌群落特征趋于一致，物种结构与泾河相似而与黑河存在明显差异.

• Abstract：The variation of the community structure of planktonic bacteria in Weihe River was investigated by using terminal restriction fragment length polymorphism (T-RFLP) and high-throughput sequencing in normal, dry and wet seasons. The results show that the community structure changed both spatially and seasonally, while temperal effect was more profound. Shannon indices of planktonic bacterial communities were 2.13~2.82, 2.05~2.84 and 2.61~2.91 in normal, dry and wet seasons, respectively. Bacterial communities in the normal season present the greatest spatial variation of diversity (RSD=16.75%), the lowest similarity of community structure (26.8%) and the highest predominant terminal restriction fragments (T-RF=23), while an opposite trend was found in wet season. Bacterial community diversity in Xianyang-Xi'an segment of the Weihe River was relatively lower than those in other segments. Canonical Correspondence Analysis (CCA) and Redundancy Analysis (RDA) indicated that the environmental factors influencing planktonic bacterial community varied with season, while total suspended solids (TSS) always had profound impacts on bacterial community during all these three sampling periods. A total number of 21 bacterial phyla and 26 candidate divisions were detected in the waters collected during the wet season. Proteobacteria and Bacteroidetes were the major bacterial groups and accounted for more than 75% in each sampling site. In wet season, bacterial community structures in the stems of the Weihe River illustrated slight difference, and also showed high similarity to that in the Jinghe River, but distinct difference from that in the Heihe River.

• Key words：Weihe River  microbial diversity  16S rDNA  T-rflp analysis  high-throughput sequencing

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