• 关键词：Fusarium.sp  玉米秸秆  黄瓜秸秆  番茄秸秆  HMW-PAHs

• 基金项目：河北省教育厅项目（No.Z2013058）

• 张雪娜
• 1. 河北农业大学资源与环境科学学院, 保定 071000;2. 河北省农田生态环境重点实验室, 保定 071000

• 贾海滨
• 河北农业大学资源与环境科学学院, 保定 071000

• 王伟
• 河北农业大学资源与环境科学学院, 保定 071000

• 张丽秀
• 河北农业大学资源与环境科学学院, 保定 071000

• 石维
• 河北农业大学资源与环境科学学院, 保定 071000

• 冯圣东
• 1. 河北农业大学资源与环境科学学院, 保定 071000;2. 河北省农田生态环境重点实验室, 保定 071000

• 杨志新
• 1. 河北农业大学资源与环境科学学院, 保定 071000;2. 河北省农田生态环境重点实验室, 保定 071000

• 摘要：本研究选用玉米、黄瓜和番茄秸秆作为载体材料，固定化PAHs高效降解菌ZH-H2（Fusarium.sp），通过土壤培养试验对煤矿区农田高环多环芳烃（HMW-PAHs）污染老化土壤进行修复研究.结果表明，土壤培养60 d后，3种材料固定化ZH-H2处理（Y+B，H+B，X+B）对10种HMW-PAHs总量均有一定的修复效果，其降解率分别为21.65%、16.10%和15.49%，且Y+B处理的修复效果高于其他两种材料处理（p<0.05）；3种载体材料固定化ZH-H2对土壤HMW-PAHs不同环数的降解存在一定的差异，其对6环PAHs的修复效果均优于4环和5环；3种材料固定化ZH-H2对Flt、Pyr、BaA、Chry、BbF、BaP 6种单体的降解也存在明显的不同.对Flt、Pyr、BaA、Chry、BbF、BaP的修复效果表现为Y+B处理显著高于H+B和X+B处理，且Y+B处理中尤其对BbF（24.20%）、InP（24.24%）的修复效果表现突出；对BghiP单体的修复效果表现为X+B处理显著高于Y+B和H+B，高达30.95%；3种处理对BkF、DbA和InP 3种PAHs单体的修复效果无差异显著性.进一步分析与HMW-PAHs降解有关的土壤酶活性发现，土壤木质素过氧化物酶和过氧化氢酶活性在3种秸秆材料处理下呈现了较为相似的规律，均表现为Y+B处理下最高，与固定化ZH-H2对Flt、Pyr、BaA、Chry、BbF、BaP 6种单体PAHs的修复效果规律相一致；而对于多酚氧化酶活性却呈现了相反的规律，表现为Y+B处理下最低的变化特征.综上，对于煤矿区农田污染老化土壤中10种PAHs总量的修复以玉米秸秆作为菌株ZH-H2的固定化载体材料最为适宜.

• Abstract：A soil incubation experiment was carried out to investigate the effect of different carrier materials for Fusarium. sp ZH-H2 immobilization on the remediation of HMW-PAHs in long-term polycyclic aromatic hydrocarbons (PAHs) polluted soils in coal mining areas. Materials of corn, cucumber, and tomato straws were considered in the present study. After 60-day soil incubation, three carrier materials (Y+B, H+B, X+B) with immobilized Fusarium.sp played positive roles on the removal of a total amount of 10 HMW-PAHs, where the 10-PAHs removals of treatment Y+B, H+B and X+B were 21.65%, 16.10% and 15.49%, respectively, and the treatment Y+B had significantly higher 10-PAHs removal than treatments of H+B and X+B (p<0.05). These three materials showed distinctive removal effects on ring-number differed HMW-PAHs, where apparently higher removals of 6-ring PAHs than 4-ring PAHs and 5-PAHs were observed. Meanwhile, for the six individual HMW-PAHs such as Flt, Pyr, BaA, Chry, BbF and BaP, significantly higher removals by treatment Y+B in contrast to treatments of H+B and X+B were found, especially for BbF (24.20%), InP (24.24%), respectively. In addition, treatment X+B showed the highest removal on BghiP (30.95%) in comparison with Y+B and H+B, however, little difference in the removals of individual BkF, DbA and InP among Y+B, H+B and X+B were observed. Further analyses of the soil enzyme activities related to HMW-PAHs removal showed that the enzyme activities of lignin peroxidase and catalase among three treatments showed similar pattern that treatment Y+B had significantly higher enzyme activities than H+B and X+B (p<0.05). This was consistent with the removals of Flt, Pyr, BaA, Chry, BbF and BaP. However, treatment Y+B showed the lowest activity of polyphenol oxidase. In conclusion, corn straw was considered to be a suitable carrier material for Fusarium.sp immobilization to phytoremediate the aged soils contaminated with ∑10PAHs in coal mining areas.

• Key words：Fusarium.sp  corn straw  cucumber straw  tomato straw  HMW-PAHs

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