• 关键词：微囊藻毒素  产毒株  环境因子  荧光定量PCR  洋河水库

• 基金项目：国家自然科学基金（No.91547206）；江苏省水利科技项目（No.2015005）

• 王敏
• 1. 南京水利科学研究院生态环境研究中心, 南京 210029;2. 中国科学技术大学生命科学学院, 合肥 230026

• 刘祥
• 南京水利科学研究院生态环境研究中心, 南京 210029

• 陈求稳
• 南京水利科学研究院生态环境研究中心, 南京 210029

• 易齐涛
• 南京水利科学研究院生态环境研究中心, 南京 210029

• 刘卓
• 南京水利科学研究院生态环境研究中心, 南京 210029

• 摘要：在洋河水库设置6个采样点，于2015年5—10月采集水样，利用高效液相色谱测定了微囊藻毒素（MC-LR、MC-RR、MC-YR）的浓度，并采用基于mcyA和mcyB基因的荧光定量PCR技术检测产毒蓝藻与产毒微囊藻种群.同时，分析了水体中微囊藻毒素浓度和组成的时空分布特征，以及产毒蓝藻与产毒微囊藻丰度的动态变化.结果表明，洋河水库微囊藻毒素（Microcystins，MCs）浓度范围为0.24~10.99 μg·L^-1；水体中产毒蓝藻占总蓝藻种群丰度的7%~55%，产毒微囊藻占总微囊藻种群丰度的8%~59%，是主要的产毒蓝藻；微囊藻毒素浓度与产毒株种群丰度时空分布特征一致，季节变化规律为夏季>秋季>春季，空间分布表现为西洋河口附近区域高于东洋河口附近区域；而微囊藻毒素组成无显著时空差异，均以MC-RR和MC-LR 2种亚型为主.Pearson相关分析显示，产毒微囊藻种群丰度及其比例是影响微囊藻毒素浓度变化的主要因素，且其与总磷和叶绿素a浓度呈显著正相关（p<0.01）.

• Abstract：Water samples were collected from six sites located in the Yanghe Reservoir of Qinhuangdao city to characterize the spatio-temporal distribution and dynamics of microcystins and microcystin-producing cells from May to October in 2015. The concentrations of MC-RR, MC-LR and MC-YR were analyzed using high-pressure liquid chromatography (HPLC). And the abundance of microcystin-producing cyanobacteria and Microcystis spp. were subsequently detected by real-time PCR assays, targeting the microcystin synthetase gene mcyA and mcyB respectively. The results show that the concentration of microcystins ranged from 0.24 to 10.99 μg·L^-1 during the study period. The ratio of toxic cyanobacteria to total cyanobacteria population, and toxic Microcystis to total Microcystis population ranged in 7%~55%, and 8%~59% respectively. Microcystis was the dominant microcystins producer in the Yanghe Reservoir. Microcystins concentration in the reservoir corresponded well with the toxic strains abundance in the spatial and temporal scale, presenting a decreased trend from summer towards spring with higher concentration and abundance occurred in west area than that in east area of the reservoir. However, the microcystins composition show no significant difference in spatio-temporal patterns, which was made of MC-LR and MC-RR. Correlation analysis by Pearson indicates that the abundance and proportion of toxic Microcystis were the determine factors affecting the variation of microcystins concentration, whose growth were correlated with the concentrations of total phosphorus and chlorophyll a in the water column (p<0.01).

• Key words：microcystins  toxic strains  environmental factors  quantitative real-time PCR  Yanghe Reservoir

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