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研究报告

Effects of high-temperature composting on tetracyclines and tetracycline resistance genes in swine manure

摘要：为了研究高温堆肥对猪粪中抗生素及抗生素抗性基因的影响，对不同堆肥处理中的四环素类抗生素（四环素、土霉素、金霉素）及其抗性基因（tetA、tetC、tetG、tetM、tetQ、tetW）的动态变化进行了定量研究.结果表明：低剂量组（10 mg·kg^-1）中四环素、土霉素、金霉素的去除率分别为91%、94%、92%，高剂量组（50 mg·kg^-1）中四环素、土霉素、金霉素的去除率分别为60%、62%、71%.在堆肥过程中，抗生素处理组目标基因相对丰度高于对照组，而低剂量组目标基因相对丰度（tetW除外）又普遍高于高剂量组.经堆肥处理后，tetA的相对丰度增加，tetC、tetG、tetM、tetQ、tetW的相对丰度均下降.高温堆肥可以有效去除猪粪中一定浓度的四环素类抗生素，并对四环素类抗性基因也有一定的削减作用.

Abstract：This experiment was designed to explore the effects of high-temperature composting on tetracyclines and tetracycline resistance genes in swine manure. The concentrations of tetracyclines (tetracycline (TC), chlortetracycline (CTC), oxytetracycline (OTC) and the abundance of tetracycline resistance genes (tetA, tetC, tetG,tetM, tetQ and tetW) were quantified during composting of swine manure with different treatments. The results show that the concentrations of TC, OTC and CTC decreased by 91%, 94% and 92% in low level group (10 mg·kg^-1), and decreased by 60%, 62% and 71% in high level group (50 mg·kg^-1) after composting respectively. During the composting, the relative abundance of target resistance genes in antibiotics treatment groups were higher than the control group, and the relative abundance of target resistance genes (except tetW) in the low level group was higher than those in the high level group. Compared with the initial composting mass, the relative abundance of tetA increased and the relative abundance of tetC, tetG, tetM, tetQ and tetW decreased after composting. In conclusion, high-temperature composting is able to reduce the concentration of antibiotics and impair the abundance of antibiotics resistance genes in swine manure.