研究报告
徐相龙,刘国华,范强,王园园,王皓,宋新新,齐鲁,王洪臣.SBR反应器厌氧氨氧化系统启动过程中细菌群落结构的变化[J].环境科学学报,2017,37(5):1695-1703
SBR反应器厌氧氨氧化系统启动过程中细菌群落结构的变化
- The shift of bacterial community structure during start-up of anaerobic ammonia oxidation (ANAMMOX) in a sequencing batch reactor
- 基金项目：中国人民大学科学研究基金（中央高校基本科研业务费专项资金资助）项目成果（No.15XNLD04）
- 作者
- 单位
- 徐相龙
- 中国人民大学环境学院, 北京 100872
- 刘国华
- 中国人民大学环境学院, 北京 100872
- 范强
- 中国人民大学环境学院, 北京 100872
- 王园园
- 中国人民大学环境学院, 北京 100872
- 王皓
- 中国人民大学环境学院, 北京 100872
- 宋新新
- 中国人民大学环境学院, 北京 100872
- 齐鲁
- 中国人民大学环境学院, 北京 100872
- 王洪臣
- 中国人民大学环境学院, 北京 100872
- 摘要：以白洋淀岸边带沉积物为接种污泥，启动了SBR厌氧氨氧化反应器.对反应器启动过程中的进出水水质进行了连续监测，并采用PCR-DGGE、定量PCR和基因测序等分子生物学技术研究了系统内总细菌和厌氧氨氧化（ANAMMOX）细菌群落结构随培养时间的变化规律.结果表明：在启动过程中，总微生物菌群动态变化水平为26.6%~50.5%；微生物多样性先变小后增大，优势菌种得到重新分布；ANAMMOX细菌的群落结构变得单一化，最后系统的优势ANAMMOX细菌是Brocadia属.富集培养阶段SBR系统中ANAMMOX细菌的最大生物量达到了1.73×109 copies·g-1干污泥，而且总氮的去除率最高达到约82%.
- Abstract：The start-up of anaerobic ammonia oxidation (ANAMMOX) system in a sequencing batch reactor (SBR), where Baiyangdian Lake shore sediments were inoculated. The microbial community structure of total bacteria and ANAMMOX bacteria in the system along with the enrichment time was investigated by using molecular biology methods (e.g. PCR-DGGE, qPCR, and DNA sequencing). Meanwhile, the water quality of the influent and effluent was continuously monitored during the whole start-up stage. The results showed that the dynamic change of total bacterial community along with the enrichment time increased from 26.6% to 50.5%, and their diversity decreased first and then increased afterwards. The ANAMMOX bacterial community diversity in the SBR became single at the end of enrichment, and was predominantly Candidutus Brocadia. The maximum biomass of ANAMMOX bacteria achieved 1.73×109 copies·g-1 dry sludge during the enrichment, with the highest removal rate of TN around 82%.
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