研究报告
姚玲丹,王丽晓,朱亮,徐向阳,楼莉萍.一株壬基酚降解菌的分离鉴定及其对壬基酚同分异构体降解特性研究[J].环境科学学报,2017,37(5):1674-1680
一株壬基酚降解菌的分离鉴定及其对壬基酚同分异构体降解特性研究
- Isolation and identification of a NP-degrading strain and its degradation characteristics for NP isomers
- 基金项目：国家自然科学基金面上项目（No.41371447）
- 作者
- 单位
- 姚玲丹
- 1. 浙江大学环境与资源学院, 杭州 310029;2. 慈溪市留学人员创业服务中心, 慈溪 315302
- 王丽晓
- 1. 浙江大学环境与资源学院, 杭州 310029;3. 浙江省水体污染控制与环境安全技术重点实验室, 杭州 310029
- 朱亮
- 浙江大学环境与资源学院, 杭州 310029
- 徐向阳
- 浙江大学环境与资源学院, 杭州 310029
- 楼莉萍
- 1. 浙江大学环境与资源学院, 杭州 310029;3. 浙江省水体污染控制与环境安全技术重点实验室, 杭州 310029
- 摘要：壬基酚（NP）是典型的内分泌干扰物，具有雌激素效应.目前对NP的研究主要集中于微生物降解对其整体去除效果，而对各NP同分异构体的降解行为尚缺乏系统性研究.从钱塘江沉积物中筛选得到一株能够降解NP的细菌，命名为N-1，实验室条件下该菌能以NP为唯一碳源生长.经形态、生理生化及16S rRNA基因序列分析，N-1鉴定为假黄单胞菌（Pseudoxanthomonas sp.）.通过正交试验确定N-1降解NP的最适条件为：温度30℃，pH=7.0，菌量10%，NP浓度5~10 mg·L-1.最适条件下，N-1对NP 16 d整体降解率可达88.0%，对10种NP异构体降解率在69.7%~100%之间，说明N-1对NP同分异构体的降解具有结构-降解特性，即不同结构NP异构体表现出不一样的降解能力.进一步研究发现，NP异构体降解率随着NP烷基C链长度增加而升高，随着烷基取代基结构越复杂而降低.NP降解选择性导致的难降解成分残留现象，为NP类异构体污染物的微生物修复提供了一定的理论依据.
- Abstract：Nonylphenol (NP) is a typical endocrine disrupting compound with estrogen effects. Recent studies mostly concentrate on the overall removal efficiency of NP, while the degradation behavior of NP isomers is still lack of systematic research. In this study, a NP-degrading strain was isolated from the sediment collected from Qiantang River, named N-1, which was able to utilize NP as a sole carbon source for growth. The morphology observation, physiological, biochemical and 16S rRNA sequence analysis revealed that N-1 belonged to Pseudoxanthomonas sp..By orthogonal experiment, the optimum conditions for NP degradation were 30℃ pH=7.0,inoculum size of 10% and NP concentration 5~10 mg· L-1.Under these conditions, the degrading rates reached 88.0% and 69.7%~100% of NP and NP isomers during 16 days of incubation, respectively, indicating that degradation of NP isomers was clearly isomer-specific, indicating degradability of NP isomers is structure dependent. Furthermore, the degradability of NP isomers was positively correlated with side chain length, while negatively correlated with the complexity of alkyl substituent. It was reasonable to speculate that the refractory composition of NP isomers may cause residue because of isomer-specific degradation, which may help providing theoretical foundation for microbial remediation in isomers of contaminants like NP.
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