• 关键词：铜绿假单胞菌  重金属  生物吸附  相互影响

• 基金项目：江苏省自然科学基金青年基金（No.BK20150287）；苏州科技学院青年基金（No.XKQ201415）；国家自然科学基金（No.31570515）

• 史广宇
• 苏州科技大学环境科学与工程学院, 苏州 215009

• 程媛媛
• 苏州科技大学环境科学与工程学院, 苏州 215009

• 史绮
• 苏州科技大学环境科学与工程学院, 苏州 215009

• 施维林
• 苏州科技大学环境科学与工程学院, 苏州 215009

• 摘要：研究了铜绿假单胞菌（Pseudomonas aeruginosa）对Cu和Pb的吸附特性.结果表明，相同条件下，该菌株对Cu²⁺的吸附率低于Pb²⁺.对于单一重金属体系，吸附率均随时间的延长先上升后平稳变化，2 h达到稳定.吸附率随投菌量的增加先迅速增加，之后趋于平稳.对于Cu²⁺，投菌量为1 g·L^-1时吸附率达到稳定，而Pb²⁺的吸附效果达到平稳时的投菌量为0.5 g·L^-1.单位质量菌体对Cu²⁺、Pb²⁺的吸附量随投菌量的增加而下降.pH为3时，菌体的吸附效果较差，当pH为5~8时，2种重金属的吸附效果较高.对于活菌，Pb²⁺对菌体吸附Cu²⁺有抑制作用，而Cu²⁺对菌体吸附Pb²⁺的影响无明显规律.对于失活菌，P.aeruginosa吸附Pb²⁺和Cu²⁺的效果均随共存重金属浓度的增大而降低，但Cu²⁺对Pb²⁺的影响比Pb²⁺对Cu²⁺的影响更显著.扫描电镜观察发现，吸附后的菌体较吸附前聚集性更好.总体而言，P.aeruginosa能对水体中共存的Cu²⁺和Pb²⁺有较好的吸附效果.

• Abstract：The biosorption characteristics of Cu and Pb was investigated in this paper. Results reveal that the adsorption rate of Cu²⁺ was lower than Pb²⁺ under the same conditions. In the presence of only one heavy metal, adsorption rate gradually increased then stabilized after 2 h. Adsorption of heavy metal was ascended rapidly with the increase of inoculum size and then tended to stabilize at inoculum dosage of 1 g·L^-1 for Cu²⁺ and 0.5 g·L^-1 for Pb²⁺.Adsorbed amount of Cu²⁺ and Pb²⁺ by unit cell was both decreased with the increase of inoculum dosage. Cu²⁺ and Pb²⁺ adsorption rate reached maximumat pH 5~8 but lowest at pH 3. As for the living stain, adsorption of Cu²⁺was inhibited when Pb²⁺ was present, but Cu²⁺ seemed to have no significant influence on the adsorption of Pb²⁺. As for inactivation of microbes, adsorption rate of Cu²⁺ and Pb²⁺ by P. aeruginosa both decreased with the increasing concentrations of heavy metals but the influence of Cu²⁺ on Pb²⁺ adsorption was more significant than Pb²⁺ for Cu²⁺ adsorption. SEM results show that the aggregation of cells was better after adsorption. Overall, P. aeruginosa shows good adsorption performance for both Cu²⁺ and Pb²⁺.

• Key words：Pseudomonas aeruginosa  heavy metal  biosorption  interaction effect

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