研究报告
刘志进,段晋明,王莉瑛.红球菌LSJ-6介导的金纳米颗粒的合成及其对亚甲基蓝的吸附去除[J].环境科学学报,2017,37(6):2092-2098
红球菌LSJ-6介导的金纳米颗粒的合成及其对亚甲基蓝的吸附去除
- Bio-synthesis of Gold nanoparticles by Rhodococcus sp. LSJ-6 and its application in methylene blue adsorption
- 基金项目：国家自然科学基金项目（No.41503094）
- 作者
- 单位
- 刘志进
- 1. 西安建筑科技大学环境与市政学院, 西安 710055;2. 中国科学院生态环境研究中心, 北京 100085
- 段晋明
- 西安建筑科技大学环境与市政学院, 西安 710055
- 王莉瑛
- 中国科学院生态环境研究中心, 北京 100085
- 摘要：从湖南省冷水江市的土样中分离筛选到一株对重金属具有高抗性的菌株.16S rRNA基因序列分析可知，该菌株属于红球菌属（Rhodococcus），命名为Rhodococcus sp.LSJ-6.最适生长条件为30℃，pH为7，好氧.研究发现该菌株可以介导金纳米颗粒的合成，且纳米颗粒的大小随体系pH升高（pH 5~9）而减小（15~3 nm）.当温度升高时（20~40℃），颗粒大小无显著性差异，但形貌的多样性减小.进一步研究了不同条件下合成的纳米颗粒对亚甲基蓝的吸附去除，发现在pH为9，30℃时合成的金纳米颗粒对亚甲基蓝的吸附去除率最高，可达96%.本研究为生物合成金纳米颗粒的环境应用奠定了基础.
- Abstract：A heavy-metal hyper-resistant strain was isolated from Lengshuijiang, Hunan Povince. 16S rRNA gene sequences Analysis reveals that this strain belongs to genus Rhodococcus and is named as Rhodococcus sp. LSJ-6. Its optimum growth condition was at 30℃ and pH 7 under aerobic condition. This strain can reduce chloroaurate to gold nanoparticles (Au NPs). The size of Au NPs (15~3 nm) decreased as pH increasing from 5~9, whereas no obvious size difference was observed with temperature between 20~40℃. Nevertheless, the diversity of the Au NPs shape reduced with the increase in temperature. This bio-synthesized Au NP was examined for the removal of methylene blue, and the results show that the highest adsorption reached 96% at pH 9 and 30℃. This study paved an avenue in the environmental application of bio-synthesized Au NPs.
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