研究报告
李方方,宋建,吕律,王洋洋,翟秋敏,杨志辉,柴立元.DBP高效降解菌群的富集及其降解特性研究[J].环境科学学报,2017,37(7):2579-2585
DBP高效降解菌群的富集及其降解特性研究
- Enrichment of a highly efficient DBP-degrading bacterium consortium and its degradation characters
- 基金项目：国家自然科学基金（No.41430637）；河南省高校科技创新团队支持计划资助（No.16IRTSTHN012）；河南省教育厅科学技术研究重点项目（No.16A610015）；河南省科技发展计划项目（No.152102310297）
- 作者
- 单位
- 李方方
- 河南大学资源与环境研究所, 开封 475004
- 宋建
- 河南大学资源与环境研究所, 开封 475004
- 吕律
- 河南大学资源与环境研究所, 开封 475004
- 王洋洋
- 1 河南大学资源与环境研究所, 开封 475004;2 黄河文明与可持续发展研究中心, 开封 475004
- 翟秋敏
- 河南大学资源与环境研究所, 开封 475004
- 杨志辉
- 中南大学冶金与环境学院, 长沙 410083
- 柴立元
- 中南大学冶金与环境学院, 长沙 410083
- 摘要：以邻苯二甲酸二丁酯（DBP）为唯一碳源及能源,从开封市垃圾焚烧发电厂活性污泥中富集得到细菌菌群LV-1,高通量测序显示LV-1共包含33个科、48个属,其中优势菌属为布鲁氏杆菌（Brucella sp.,62.78%）和中华杆菌（Sinobacter sp.,14.83%）.在最佳降解条件下（30 ℃、pH 6.0）,LV-1 48 h内可将500 mg·L-1 DBP降解93%左右,72 h内将1000 mg·L-1 DBP降解95%以上.当DBP浓度为100~500 mg·L-1时,LV-1对DBP的降解过程符合一级动力学方程,半衰期为13.64~19.20 h.利用GC-MS对DBP生物降解的中间产物进行分析,推测LV-1降解DBP的生化途径：DBP→邻苯二甲酸-1-乙基-6-丁基酯（BEP）→邻苯二甲酸单丁酯（MBP）→邻苯二甲酸单乙酯（MEP）→邻苯二甲酸（PA）→CO2+H2O.此外,LV-1还能够有效降解侧链较短的邻苯二甲酸二甲酯（DMP）、邻苯二甲酸二乙酯（DEP）及PA.
- Abstract：A bacteria consortium (LV-1), which can utilize di-n-butyl phthalate (DBP) as sole source of carbon and energy, was isolated from activated sludge collected from a garbage incineration power plant of Kaifeng. Based on the results of Illumina sequencing, LV-1 contains 33 families and 48 genera of bacteria, the dominant genera is Brucella sp. and Sinobacter sp. and their relative abundance is 62.78% and 14.83%, respectively. The optimal degradation condition of LV-1 to degrade DBP was: temperature 30 degrees centigrade and pH 6.0. Under the optimum conditions, LV-1 degraded about 93% of 500 mg·L-1 DBP within 48 h, and more than 95% of 1000 mg·L-1 DBP was degraded within 72 h. The investigation of degradation kinetics at different DBP initial concentrations (100~500 mg·L-1) showed that DBP degradation fitted the Monod first-order kinetic equation, and the half-life ranged from 13.64 to 19.20 h. Gas chromatography-mass spectrometry (GC-MS) was used to analysis the intermediates during the DBP degradation process, and the degradation pathway was proposed: DBP→BEP→MBPMEP→PA→CO2 + H2O.Besides, LV-1 can effectively utilize DMP, DEP and PA.
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