研究报告
叶杰旭,诸葛蕾,蔡武,陈东之,陈建孟.甲硫醇降解菌群筛选及其降解特性研究[J].环境科学学报,2017,37(7):2572-2578
甲硫醇降解菌群筛选及其降解特性研究
- Enrichment and characterization of a methanthiol-degradating mixed microbial consortium
- 基金项目：国家自然科学基金青年基金（No.51308502）；浙江省公益技术应用研究项目（No.2015C33037）；浙江省教育厅项目（No.Y201329224）
- 作者
- 单位
- 叶杰旭
- 浙江工业大学环境学院, 杭州 310032
- 诸葛蕾
- 浙江工业大学环境学院, 杭州 310032
- 蔡武
- 浙江工业大学环境学院, 杭州 310032
- 陈东之
- 浙江工业大学环境学院, 杭州 310032
- 陈建孟
- 浙江工业大学环境学院, 杭州 310032
- 摘要：从处理甲硫醚（DMS）和丙硫醇（PT）混合废气的生物滴滤塔中富集出一组能够有效降解甲硫醇（MT）的混合菌群,并对其特性进行了系列研究.结果表明,该混合菌群能有效降解MT,菌群较为适宜的生长和降解条件为30 ℃、pH=7.0,在该条件下能将初始浓度为20 mg·L-1的MT在70 h内降解完全.添加酵母膏（YE）后,MT降解速率进一步提高,降解所需时间缩短10 h.利用高通量测序技术分析混合菌群的群落结构,发现其中优势菌属为Pseudomonas sp.、Thiobacillus sp.和Acinetobacter sp.,所占比例分别为33.78%、21.91%和17.01%.中间产物检测结果表明,混合菌群降解MT的过程中产生了甲醛、H2S、二甲基二硫醚（DMDS）等物质,推断MT的降解途径可能有如下2条：1MT在MT氧化酶作用下形成甲醛和H2S,随后氧化为SO42-；2MT依次转化为DMDS、DMS、二甲基亚砜（DMSO）和二甲基砜（DMSO2）,最后经甲基磺酸（MSA）可生成SO42-.
- Abstract：A mixed microbial consortium which could utilize methanthiol (MT) as sole carbon and sulfur source was enriched from biofilm of a biotrickling filter treating dimethyl sulfide (DMS) and 1-propanethiol (PT). Experimental results show that the mixed microbial consortium achieved a high degradation rate of MT. Under the optimized conditions under pH=7.0 and the operation temperature of 30 ℃, 20 mg·L-1 of MT could be completely degraded within 70 hours. Moreover, the addition of the yeast extract (YE) significantly enhanced MT degradation, with shorter time (60 hours) for complete degradation. High-throughput sequencing were used to identify the community structure of the mixed microbial consortium. The results indicate that Pseudomonas sp. (33.78%), Thiobacillus sp. (21.91%), and Acinetobacter sp. (17.01%) were the dominating bacteria. Furthermore, the intermediates of MT degradation were detected by using GC, GC-MS, and HPLC. Based on the intermediates and the final products, two metabolic pathway of MT were inferred,1MT was firstly converted to formaldehyde and H2S and finally oxidized to sulfate,2MT oxidation to sulfate occurs via successive intermediates of DMDS, DMS, DMSO and DMSO2.
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