研究报告
唐超,喻慧,杨传俊,蔡澎.电磁环境胁迫对铜绿微囊藻氧化应激和光合固碳酶的影响作用研究[J].环境科学学报,2017,37(8):3194-3200
电磁环境胁迫对铜绿微囊藻氧化应激和光合固碳酶的影响作用研究
- The effects of the electromagnetic environment on oxidative stress and photosynthetic carbon fixation of Microcystis aeruginosa
- 基金项目：中国科学院国防科技创新基金（No.CXJJ-16M115）；厦门科技计划项目（No.3502Z20162021）
- 作者
- 单位
- 唐超
- 1. 中国科学院城市环境研究所, 中国科学院城市环境与健康重点实验室, 厦门 361021;2. 中国科学院大学, 北京 100049
- 喻慧
- 中国科学院城市环境研究所, 中国科学院城市环境与健康重点实验室, 厦门 361021
- 杨传俊
- 中国科学院城市环境研究所, 中国科学院城市环境与健康重点实验室, 厦门 361021
- 蔡澎
- 中国科学院城市环境研究所, 中国科学院城市环境与健康重点实验室, 厦门 361021
- 摘要：研究了3种典型电磁环境（射频电磁辐射（RF-EMF）、极低频电磁场（ELF-EMF）、交变电场（AEF））胁迫对铜绿微囊藻（Microcystis aeruginosa）细胞氧化应激（超氧化物歧化酶（SOD）、丙二醛（MDA））和光合固碳酶（核酮糖-1，5-二磷酸羧化酶/加氧酶（Rubisco）、果糖-1，6-二磷酸醛缩酶（FBA））的影响作用.结果表明，短期和长期RF-EMF、ELF-EMF及AEF暴露胁迫铜绿微囊藻细胞，总体能极显著提高藻细胞的SOD活性（p<0.01）.铜绿微囊藻经RF-EMF、ELF-EMF及AEF短期暴露处理后，均极显著提高了藻细胞内MDA含量（p<0.01）；铜绿微囊藻经RF-EMF、AEF长期暴露处理后，藻细胞内MDA含量极显著降低（p<0.01）.RF-EMF暴露处理铜绿微囊藻，总体能显著提高藻细胞Rubisco酶活性（p<0.05）.长期RF-EMF、AEF和ELF-EMF暴露下的铜绿微囊藻，总体表现出FBA酶活性极显著降低（p<0.01）.以上结果说明，RF-EMF、ELF-EMF、AEF均能够诱导铜绿微囊藻发生氧化应激反应.而RF-EMF能够对铜绿微囊藻细胞参与光合作用的关键酶（Rubisco和FBA）产生一定的调控作用，表明射频电磁波能够在一定程度上影响铜绿微囊藻细胞的光合作用.
- Abstract：The effect of the activities of antioxidant enzyme systems (SOD, MDA) and photosynthesis catalytic enzymes (Rubisco, FBA) of Microcystis aeruginosa were studied under the exposure of three kinds of typical electromagnetic environment (radiofrequency electromagnetic fields(RF-EMF), extremely low frequency electromagnetic fields(ELF-EMF), alternating electric field(AEF)). The results showed that the activities of SOD in M. aeruginosa cells increased significantly under the RF-EMF, ELF-EMF and AEF under both short and long term (p<0.01). The contents of MDA in M. aeruginosa cells increased significantly under the short term exposure of RF-EMF, ELF-EMF, and AEF (p<0.01). The contents of MDA in M.aeruginosa cells decreased significantly under the sustainable exposure of RF-EMF and AEF (p<0.01). The activity of Rubisco in M.aeruginosa cells increased significantly under the exposure of RF-EMF(p<0.05). The long term exposure of RF-EMF, AEF and ELF-EMF to M.aeruginosa significantly reduced FBA activity(p<0.01). The results mentioned above indicated that oxidative stress of M.aeruginosa could be induced under the exposure of RF-EMF, ELF-EMF and AEF. And the regulations on key enzymes of photosynthesis (Rubisco and FBA) by RF-EMF implied that radiofrequency electromagnetic fields could affect the photosynthesis of M. aeruginosa cells to some extent.
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