研究报告
袁开,吴佳佳,朱诚.养殖鱼塘底泥微生物抗生素耐药基因分布分析[J].环境科学学报,2017,37(10):3649-3655
养殖鱼塘底泥微生物抗生素耐药基因分布分析
- Analysis of microbial antibiotic resistance genes in sediment of aquaculture pond
- 基金项目：国家自然科学基金（No. 31601464）；浙江省自然科学基金项目（No.LQ15C200001）
- 作者
- 单位
- 袁开
- 1. 中国计量大学生命科学学院, 杭州 310018;2. 浙江省海洋食品品质及危害物质控制技术重点实验室, 杭州 310018
- 吴佳佳
- 1. 中国计量大学生命科学学院, 杭州 310018;2. 浙江省海洋食品品质及危害物质控制技术重点实验室, 杭州 310018
- 朱诚
- 1. 中国计量大学生命科学学院, 杭州 310018;2. 浙江省海洋食品品质及危害物质控制技术重点实验室, 杭州 310018
- 摘要：针对水产养殖中抗生素滥用引起微生物耐药基因污染的现象，本实验以杭州市某水产养殖区底泥样品为研究对象，测定了底泥样品中抗生素含量，并从其中分离出74株可培养细菌.采用PCR方法对分离菌株中磺胺类、四环素类、喹诺酮类、氯霉素类抗性基因（antibiotic resistance genes，ARGs）和整合子基因进行了检测.结果表明，除tetA（四环素类）耐药基因未被检出外，其余3种耐药基因及整合子基因均被检出，其中qnrS（喹诺酮类）耐药基因的检出率最高，为50.00%.另外，利用16S rDNA序列分析技术将分离菌株鉴定为12个属、19个种，包括环境中多种常见土著细菌及部分致病菌，其中气单胞菌（Aeromonas spp.）数量最多（29株），占分离菌株的39.19%.掌握水产养殖区中ARGs的污染现状，对控制其传播、保障食品安全和保护微生态环境具有重要指导意义.
- Abstract：Antibiotics abuse in aquaculture industry has been promoting the spread of antibiotic resistance genes (ARGs). The aim of the study presented here was to investigate the ARGs pollution status in the aquaculture environment of Hangzhou area. Sediment samples were collected from the fish pond. The concentrations of sulfonamide, tetracycline, quinolone and chloramphenicol were checked initially and 74 bacterial isolates were picked up from the samples. The antibiotic resistance-related genes corresponding to the testing antibiotics within all the isolates were amplified, and the PCR results indicated that sulⅡ, qnrS, floR and the integrase encoding gene (intI) of class 1 integrons could be detected in the isolates, with the highest detection rate of 50.00% for qnrS. No tetA gene was detected. Furthermore, based on 16S rDNA sequencing analysis, the bacterial isolates were classified into 12 genera and 19 species, including a variety of indigenous bacteria in the environment as well as some pathogenic bacteria. Among them, Aeromonas spp. were the predominant population with the largest number of 29 (accounting for 39.19% of isolates). Comprehensive understanding of the ARGs pollution status in aquaculture environment is of great importance to ARGs transmission control, food safety and micro-ecological protecting.
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