• 关键词：胞外多聚物  废旧锌锰电池  生物浸提  三维荧光  Fe³⁺/Fe²⁺  机理研究

• 基金项目：陕西省高水平大学专项资金（No.2013SXTS03）；延安市科技创新团队（No.2015CHTD-04）；延安大学博士科研启动项目（No.YDBK2017-03）；延安大学引导项目（No.YDY2017-10）；陕西省、延安大学大学生创新创业训练计划项目（No.1546，D2016028）

• 牛志睿
• 延安大学石油工程与环境工程学院, 延安 716000

• 祁乙浩
• 延安大学石油工程与环境工程学院, 延安 716000

• 史宁
• 延安大学石油工程与环境工程学院, 延安 716000

• 刘强
• 延安大学石油工程与环境工程学院, 延安 716000

• 摘要：以A.thiooxidans和L.ferrooxidans为浸提菌株，研究其胞外多聚物（EPS）在废旧锌锰电池生物浸提中的作用.通过三维荧光获取了生物浸提中EPS的组成及特征变化，并利用半透膜包覆生物淋滤实验和扫描电镜（SEM）、透射电镜（TEM）等手段探讨了菌株及其EPS与电极材料的界面行为.结果表明：EPS促进了菌株与材料的接触，强化了Fe³⁺/Fe²⁺的生物循环；EPS总荧光区域积分标准体积（Φ_T，n）与锌、锰的溶出效率之间拟合的决定系数分别为0.942和0.981，EPS保有量显著提升了锌、锰浸提效率；废旧锌锰电池溶释是菌株EPS参与下的直接接触浸提及其代谢产物间接浸提的共同结果.

• Abstract：The function of extracellular polymeric substances (EPS) in bioleaching process of the spent Zn-Mn batteries have been examined with A. thiooxidans and L. ferriphilum as leaching strains. The composition and characteristics of ESP varied during bioleaching pocess have been studied by using three-dimension fluorescence spectra. The interface behavior among bacteria, EPS and electrode materials have been discussed by the experiment of bioleaching with semi-permeable membrane bag, scanning electron microscopy (SEM) and transmission electron microscopy (TEM). The results have shown that EPS has promoted the contact between bacteria and battery materials and a cycle reaction of Fe³⁺/Fe²⁺. The determination coefficients of the dissolution efficiency of Zn/Mn and excitation-emission area volumes (Φ_T,n) were 0.942 and 0.981 respectively, and the efficiency of bioleaching of zinc and manganese have been enhanced by the amount of EPS. The spent Zn-Mn batteries dissolution is the result from interactions among direct leaching of bacteria ESP and indirect leaching of its metabolites.

• Key words：extracellular polymeric substances  spent Zn-Mn batteries  bioleaching  3-D fluorescence analysis  Fe³⁺/Fe²⁺  mechanism exploration

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