研究报告
蔡敬,赵陆敏,黄旭雄,穆亮亮.不同氮磷比条件下绿球藻对猪场污水的净化效率[J].环境科学学报,2017,37(10):3696-3701
不同氮磷比条件下绿球藻对猪场污水的净化效率
- Purification efficiency of swine wastewater by Chlorococcum sp. with different N:P ratios
- 基金项目：上海市科技兴农项目[沪农科攻字（2015）第1-2号]
- 作者
- 单位
- 蔡敬
- 上海海洋大学农业部淡水水产种质资源重点实验室, 上海 201306
- 赵陆敏
- 上海海洋大学农业部淡水水产种质资源重点实验室, 上海 201306
- 黄旭雄
- 1. 上海海洋大学农业部淡水水产种质资源重点实验室, 上海 201306;2. 上海市水产养殖工程技术研究中心, 上海 201306;3. 水产动物遗传育种中心上海市协同创新中心(ZF1206), 上海 201306
- 穆亮亮
- 上海海洋大学农业部淡水水产种质资源重点实验室, 上海 201306
- 摘要：采用添加NaH2PO4的方法调节猪场污水中氮磷比（N：P）分别为8：1、16：1、32：1和64：1，以未添加NaH2PO4的污水为对照（氮磷比为532：1），探讨一株耐污绿球藻（Chlorococcum sp.）在不同氮磷比污水中的生长性能及其对猪场污水（初始氨氮浓度为291.31 mg·L-1）的净化效果.结果表明：经过12 d的培养，绿球藻（接种密度为400×104 cells·mL-1）在N：P为64：1的污水中生长最好，且对污水中氨态氮和总氮的去除效果最佳，细胞密度和生物量分别为3393×104 cells·mL-1和0.49 g·L-1，对氨态氮和总氮的去除率分别为74.94%和48.78%，显著高于对照组，氨态氮浓度降低到73.01 mg·L-1，总氮浓度降低到148.96 mg·L-1.培养期间各试验组污水中硝态氮浓度均升高.培养12 d后， N：P为64：1组污水中总磷浓度降低为3.07 mg·L-1，去除率为71.86%.综上，绿球藻在N：P为64：1的污水中生长性能及其对污水中氨态氮和总氮的去除效果均最佳，可使污水中的氨态氮和总磷浓度基本达到相关排放标准.
- Abstract：The present study was conducted to investigate the growth and purification efficiency of swine wastewater (initial ammonia nitrogen content of 291.31 mg·L-1) by microalgae Chlorococcum sp. under different N/P (8:1, 16:1, 32:1 and 64:1), mediated by supplementation of NaH2PO4.After 12 days' cultivation, Chlorococcum sp. (inoculated 400×104 cells·mL-1) cultured in swine wastewater with N:P of 64:1 obtained the highest growth with the maximum cell density of 3393×104 cells·mL-1 and biomass concentration of 0.49 g·L-1. The concentrations of ammonia nitrogen and total nitrogen decreased to 73.01 mg·L-1 and 148.96 mg·L-1, respectively, with removal rates of 74.94% and 48.78%, respectively, which were significantly higher than those of the control. However, the nitrate concentration in all treatments increased during the cultivation. Also, the concentration of total phosphorus in the group with N:P of 64:1 decreased to 3.07 mg·L-1 with removal rate of 71.86% after 12 days' cultivation. In summary, Chlorococcum sp. displayed the best growth performance and efficient purification of ammonia and total nitrogen in swine wastewater under N:P ratio of 64:1, and the contents of ammonia nitrogen and total nitrogen in the swine wastewater would generally reach the discharge standards after purification.
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