• 关键词：农作物秸秆  纤维素降解菌  纤维素酶  酶活  酶学性质

• 基金项目：国家自然科学基金（No.31070087）

• 万文结
• 中南民族大学生命科学学院, 武汉 430074

• 刘月
• 中南民族大学生命科学学院, 武汉 430074

• 薛芷筠
• 中南民族大学生命科学学院, 武汉 430074

• 张泽文
• 中南民族大学生命科学学院, 武汉 430074

• 程国军
• 中南民族大学生命科学学院, 武汉 430074

• 李晓华
• 中南民族大学生命科学学院, 武汉 430074

• 何冬兰
• 中南民族大学生命科学学院, 武汉 430074

• 摘要：采用单一碳源选择性培养基和纤维素平板水解圈法筛选到一株具有较强纤维素分解能力的菌株Arthrobacter oryzae HW-17.此外，高通量测序发现，不同驯化条件下微生物群落结构有明显差异，低温条件下优势属为类芽孢杆菌属（Paenibacillus）和伯克氏菌属（Burkholderia）.本文同时对菌株Arthrobacter oryzae HW-17的微生物特性和纤维素降解特性进行了初步研究，结果发现，KNO₃、30或35℃、pH=7分别为菌株产纤维素酶的最佳氮源、温度和pH.菌株HW-17的最高纤维素酶活为18.55 U·mL^-1，且对磨碎加工处理的纤维素样品和含鸡粪的纤维素混合样品有更好的降解效果.此外，菌株HW-17产生的纤维素酶在中温（≤ 50℃）和偏酸性（pH=5~7）条件下能保持较高的酶活.Cu（Ⅱ）和Pb（Ⅱ）等金属离子能够抑制该酶的活性.

• Abstract：In this research, a strain Arthrobacter oryzae HW-17 with strong cellulose decomposing ability was isolated by using selective medium containing single carbon source and using the method of hydrolysis of cellulose plate. Besides, high-throughput sequencing results reveal that there were significant differences of the microbial community compositions when bacteria were acclimated in different conditions, Paenibacillus and Burkholderia were the dominant genus in mesophilic conditions. In this paper, the microbial properties and cellulose degradation characteristics of strain HW-17 were investigated. The results indicated that optimum nitrogen source, temperature, and pH were KNO₃, 30℃ or 35℃, and pH=7, respectively, for the production of cellulase. The highest cellulase activity of strain HW-17 was 18.55 U·mL^-1, and strain HW-17 exhibited a better degradation performance for milled cellulose samples and mixed cellulose samples containing chicken manure. Besides, the cellulase produced by strain HW-17 could retain high enzyme activity in medium temperature (≤ 50℃) condition and weak acid (pH=5~7) condition, and its enzyme activity was inhibited by Cu(Ⅱ) and Pb(Ⅱ).

• Key words：crop straw  cellulose-decomposing bacterium  cellulase  enzyme activity  enzymatic properties

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