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喻浪风,吴平霄,陈梅青,亢春喜,刘帅,朱能武,黄柱坚.铜绿假单胞菌负载Fe(III)对水中六价铬的生物吸附性能研究[J].环境科学学报,2017,37(10):3702-3712

Biosorption of hexavalent chromium on Pseudomonas aeruginosa pretreated with Fe(III)

基金项目：国家重点研发计划（No.2017YFD0801000）；国家自然科学基金（No.41673092，41472038，41273122）；广东省科技计划项目（No.2014A020216002， 2016B020242004）；广州市科技计划项目（No.201604020064）；广东特支计划百千万工程领导人才项目（No.201626011）

吴平霄
1. 华南理工大学环境与能源学院, 广州 510006;2. 工业聚集区污染控制与生态修复教育部重点实验室, 广州 510006;3. 广东省环境风险防控与应急处置工程技术研究中心, 广州 510006;4. 广东省环境纳米材料工程技术研究中心, 广州 510006

朱能武
1. 华南理工大学环境与能源学院, 广州 510006;2. 工业聚集区污染控制与生态修复教育部重点实验室, 广州 510006;3. 广东省环境风险防控与应急处置工程技术研究中心, 广州 510006;4. 广东省环境纳米材料工程技术研究中心, 广州 510006

摘要：采用原位沉积技术将Fe（Ⅲ）负载于铜绿假单胞菌（Pseudomonas aeruginosa，简称Pa）表面制备了Fe（Ⅲ）与细菌的复合体（Fe-Pa），研究了Fe-Pa对水溶液中Cr（VI）的吸附特性，探讨了最佳合成条件、Fe-Pa投加量、溶液pH值、时间和Cr（VI）初始浓度等因素对Cr（VI）吸附效果的影响，同时利用SEM、FT-IR、XPS和Zeta电位对Fe-Pa进行表征分析.吸附实验结果显示，Fe（Ⅲ）浓度为600 mg·L^-1、细菌投加量为0.5 g·L^-1制备的Fe-Pa效果最佳；Fe-Pa去除Cr（VI）适宜于酸性条件进行；Fe-Pa对Cr（VI）的吸附速率较快，60 min内可达到吸附平衡，为自发的吸热吸附，且符合准二级动力学和Langmuir等温模型.表征结果表明，Fe（Ⅲ）成功地负载到铜绿假单胞菌上，为吸附Cr（VI）提供更多的活性位点，主要机制为静电吸附作用、络合作用和还原作用.经过4次吸附/再生后，Fe-Pa对Cr（VI）的吸附能力仍在72%以上，表明Fe-Pa具有较好的重复使用性.

Abstract：Pseudomonas aeruginosa was pretreated with Fe(Ⅲ) solution to form a biosorbent named as Fe-Pa. The adsorption behavior characteristics of Cr(VI) onto Fe-Pa in aqueous solution were investigated by evaluating optimum synthesis conditions, the efffects of biomass dosage, pH value, contact time and initial concentration of Cr(VI). The physico-chemical properties of Fe-Pa were performed by using Scanning electronic microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), X-ray photoelectron spectroscopy (XPS) and Zeta potential. The best preparation condition of Fe-Pa was under Fe(Ⅲ) concentration of 600 mg·L^-1 with P. aeruginosa dosage of 0.5 g·L^-1. The lower pH was more benefit for Cr(VI) adsorption onto Fe-Pa. Cr(VI) adsorption was a endothermic and spontaneous process and could reach equilibrium within 60 min. Pseudo-second-order kinetics model and Langmuir adsorption model fitted the experimental data well. Fe(Ⅲ) was successfully loaded onto the surface of Pseudomonas aeruginosa, which provided a large number of activated sites for the adsorption of Cr(VI). Electrostatic interactions and surface complexation were inferred to be the main mechanisms of adsorption of Cr(VI) on Fe-Pa. After 4 times of adsorption/regeneration test, the maximum removal efficiency of Cr(VI) could maintain 72%, which indicates that Fe-Pa had an excellent reusability.