研究报告
苏丹,巩春娟,王鑫,侯伟.耐冷腐殖酸吸附态PAHs降解菌筛选及其降解特性[J].环境科学学报,2017,37(10):3943-3950
耐冷腐殖酸吸附态PAHs降解菌筛选及其降解特性
- Isolation and charactrizaton of cold-resistance, PAHs-degrading bacterium
- 基金项目：国家自然科学基金（No. 41501346）；沈阳市科技计划项目（No. F16-157-9-00）
- 作者
- 单位
- 苏丹
- 辽宁大学环境学院, 沈阳 110036
- 巩春娟
- 辽宁大学环境学院, 沈阳 110036
- 王鑫
- 沈阳大学区域污染环境生态修复教育部重点实验室, 沈阳 110044
- 侯伟
- 辽宁大学环境学院, 沈阳 110036
- 摘要：为了寻找自然环境中高效PAHs降解菌，并应用于北方寒冷地区PAHs污染土壤修复，从沈抚灌渠冻融土壤中筛选出一株以腐殖酸（HA）吸附态PAHs为碳源和能源且生长良好的耐冷菌株，结合其生理生化特征和16S rDNA序列比对，鉴定此菌株为假单胞菌（Pseudomonas sp.SDR4），并研究了其对冻融土壤中HA吸附态PAHs的降解性能. 结果表明：Pseudomonas sp.SDR4对冻融土壤中Phe、Pyr和BaP均具有一定的降解能力，42 d降解率分别为73.88%、64.88%和49.39%. 加入HA能够提高其对Phe、Pyr和BaP的降解率，42 d降解率分别提高15.9%、13.8%和8.6%；在降解初期加入HA可显著提高Phe、Pyr和BaP的降解速率，第1周，降解速率分别提高37.8%、28.4%和39.1%，但在后期促进效果减弱.若要在寒冷地区选择Pseudomonas sp.SDR4作为固定化菌株，可添加HA提高其修复效果.本研究为北方寒冷地区PAHs污染土壤修复提供了新的固定化菌种.
- Abstract：In order to search for efficient PAH degrading bacteria in the natural environment, and to apply them in remediation of PAH contaminated soil in northern cold area, a cold resistant bacterium, which could use humic acid (HA) adsorbed PAHs as sole carbon and energy sources was screened from freezing-thawing soils of Shenfu Irrigation Area. This bacterium was identified as Pseudomonas sp.SDR4 with physiological-biochemical characterization and 16S rDNA sequencing analyses.its degradation ability for of HA adsorbed PAHs was studied. The results showed that this Pseudomonas sp.SDR4 can degrade phenanthrene, pyrene, and benzo(a)pyrene in the soil with 73.88%,88% and 49.39% of efficiency respectively. Addition of HA enhanced biodegradation rate of phenanthrene, pyrene and benzo(a)pyreneby 15.9%, 13.8% and 8.6% respectively. The enhancements of biodegradation occurred at the early stage of HA addition, especially in the first week. But were not so signficant in the later stage. If Pseudomonas sp. SDR4 is selected in the cold region as a immobilised strain, addition of HA can improve its remediation efficiency. This study provides new immobilized strain for remediation of PAH contaminated soil in northern cold area.
- Key words：soil in the freezing and thawing process adsorbed PAHs low temperature microorganisms screening
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