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Microbial community diversity in the combined tide flow-subsurface flow constructed wetland

摘要：为研究潮汐流-潜流人工湿地组合的微生物群落空间分布，构建了潮汐流-潜流湿地组合实验装置对人工模拟生活污水进行净化处理，采用PCR-DGGE技术对组合工艺实验装置中微生物空间分布特征进行研究.结果表明，种植植物（香蒲）的人工湿地组合NH₄⁺-N、NO₃^--N和NO₂^--N去除率分别为70.22%、28.42%和38.30%，未种植植物的人工湿地组合NH₄⁺-N、NO₃^--N和NO₂^--N去除率分别为55.15%、65.26%和61.70%.两组湿地内优势微生物种类共有44种，潮汐流单元微生物种类空间分布差异性较小，系统内多为好氧微生物.潜流人工湿地单元微生物空间分布差异较大，上层以好氧微生物为主，中下层以缺氧和厌氧微生物为主.种植植物可以提高湿地系统中微生物量、微生物群落多样性和均匀性.潮汐流-潜流组合工艺在系统内实现了硝化-反硝化的组合，比一般的潜流和表面流人工湿地组合TN去除率提高了20%~30%.

Abstract：The combined tide flow-subsurface flow constructed wetland were built to treat simulated domestic sewage. PCR-DGGE technology was used to study the spatial distribution of microbial community in wetland combinations. The results show that, the removal of NH₄⁺-N、NO₃^--N and NO₂^--N in the combined constructed wetland with plants were 70.22%, 28.42% and 38.30%, respectively, while the corresponding removal rate of NH₄⁺-N、NO₃^--N and NO₂^--N in the combined constructed wetland without plants were 55.15%, 65.26% and 61.70%. 44 kinds of dominant microorganisms were founded in wetland combinations. In tide flow unit, aerobic microorganisms account for the majority and had no significant difference with the variation of depth. In subsurface flow unit, aerobic, facultative and anaerobic microorganisms are distributed in the upper, middle and lower parts. Growing plants could better enrich the microorganisms around the substrate and increased the diversity of bacterial community. The combined constructed wetland formed the nitrification-denitrification system, enhancing the purification efficiency of TN, even 20% to 30% higher than the general hybrid constructed wetland of subsurface flow and free water surface flow system.