• 关键词：摩西管柄囊霉  阿特拉津  丛枝菌根根际  细菌群落

• 基金项目：国家自然科学基金（No.31500431，31270535）；黑龙江省普通本科高等学校青年创新人才培养计划（No.UNPYSCT-2016077）；哈尔滨市应用技术研究与开发项目（No.2017RAQXJ065，2015RQQXJ004）

• 范晓旭
• 黑龙江大学生命科学学院, 哈尔滨 150080

• 邹佳迅
• 黑龙江大学生命科学学院, 哈尔滨 150080

• 梁冰冰
• 黑龙江大学生命科学学院, 哈尔滨 150080

• 宋福强
• 黑龙江大学生命科学学院, 哈尔滨 150080

• 摘要：为了探求丛枝菌根（Arbuscular Mycorrhiza，AM）根际细菌群落对阿特拉津胁迫的响应，本试验以摩西管柄囊霉（Funneliformis mosseae）为供试AM真菌菌株，以紫花苜蓿（Medicago sativa）为宿主植物，利用盆栽法建立AM共生体后，再施加10 mg·kg^-1阿特拉津进行胁迫.当阿特拉津去除率达到50%时，分别采集未接种+未施药（NM.NA）、接种+未施药（AM.NA）、未接种+施药（NM.AT）和接种+施药（AM.AT）4种处理的根际土壤，通过16S rRNA V4高通量测序技术解析了根际土壤细菌群落特征，以及其与接种F.mosseae、施加阿特拉津之间的相关性.结果表明，变形菌门（Proteobacteria）、放线菌门（Actinobacteria）和厚壁菌门（Firmicutes）为4种处理根际土壤中的三大优势菌群.单独接种F.mosseae或施加阿特拉津均未对根际细菌群落α-多样性产生显著影响，但先接种F.mosseae形成AM后，施加阿特拉津可引起细菌群落Shannon指数显著增加.此外，接种F.mosseae提高了根际土壤中具有阿特拉津降解潜力菌属Arthrobacter、Mycobacterium、Frankia和Stenotrophomonas的相对丰度，其中，Arthrobacter受F.mosseae、阿特拉津及其二者交互作用的显著性影响.本研究结果在一定程度上揭示了AM间接提高土壤中具有阿特拉津降解潜力菌属的相对丰度，也可为利用AM真菌修复阿特拉津污染土壤奠定一定的理论基础.

• Abstract：In order to reveal response of bacterial community in arbuscular mycorrhiza (AM) rhizosphere to atrazine, the AM fungi Funneliformis mosseae and host plant Medicago sativa were used in this research. The AM symbiosis system was established in pots and then 10 mg·kg^-1 atrazine was applied. When atrazine removal rate was up to 50%, the rhizosphere soil samples were collected respectively in four treatments, including non-inoculation and non-atrazine (NM.NA), inoculation and non-atrazine (AM.NA), non-inoculation and atrazine (NM.AT), and inoculation and atrazine (AM.AT). The bacterial community characteristics in rhizosphere soil and its correlations with F. mosseae inoculation and atrazine were analyzed by high throughput sequencing technique targeting the V4 regioin of 16S rRNA. These results show that the most abundant bacteria were in Proteobacteria, Actinobacteria and Firmicutes of four treatments. There was no significant effect of solely F. mosseae or atrazine on α-diversity index of bacterial community. However, atrazine applied after AM establishment enhanced Shannon index significantly. Besides, F. mosseae inoculation increased the relative abundance of Arthrobacter, Mycobacterium, Frankia and Stenotrophomonas, which has high potential to degrade atrazine. The relative abundance of Arthrobacter was significantly affected by F. mosseae, atrazine and their interaction. In general, it is suggested that atrazine removal promoted by AM may be related to the increased relative abundance of atrazine degrading bacteria in soil. The research will be useful to provide theoretical foundation for atrazine contaminated soil remediation with AM fungi.

• Key words：Funneliformis mosseae  atrazine  arbuscular mycorrhiza rhizosphere  bacterial community

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