• 关键词：半悬浮生物填料  同步硝化反硝化  DO微电极  氧传递

• 基金项目：国家自然科学基金（No.21476050）

• 郑丽纯
• 广东工业大学, 环境科学与工程学院, 广州 510006

• 汤兵
• 广东工业大学, 环境科学与工程学院, 广州 510006

• 郑义
• 广东工业大学, 环境科学与工程学院, 广州 510006

• 丁杰伟
• 广东工业大学, 环境科学与工程学院, 广州 510006

• 李志杰
• 广东工业大学, 环境科学与工程学院, 广州 510006

• 摘要：研究了不同的C/N比对半悬浮生物填料同步硝化反硝化（SND）过程的影响，并尝试找出能够实现完全脱氮的最佳C/N比.半悬浮生物填料生物膜反应器采用一种新颖的DO微电极技术展开试验，其结果从物质传递和分子生物学角度来阐明SND效率的差别.结果表明，物质传质和微生物的因素对SND效率有联合作用，生物膜的生物量、生物膜的结构和厚度及EPS在SND过程中有着重要的作用.使用半悬浮生物填料明显提高生物膜反应器内的生物多样性，它可以在C/N比20的条件下运行8 h后实现总氮的去除.

• Abstract：This work mainly focused on the effects of different C/N ratio on the process of simultaneous nitrification-denitrification (SND) on semi-suspended bio-carriers, and tried to find a suitable C/N ratio to achieve the total removal of nitrogen. The experiment was carried out by using a novel DO microelectrode technique in a biofilm reactor packed with semi-suspended bio-carriers, whose results illustrated the difference in the SND efficiency from the viewpoint of mass transferring and molecular biology. The results indicated that the mass transferring and microbial factors had a combined effect on the efficiency of SND, in which, the biomass of biofilm, structure and thickness of the biofilm, and EPS all played an important role in the process of SND. The used semi-suspended bio-carriers obviously improved the biodiversity within the biofilm reactor, which could achieve the total removal of nitrogen under the C/N ratio of 20 after operating for 8 h.

• Key words：semi-suspended bio-carrier biofilm  simultaneous nitrification and denitrification (SND)  DO microelectrode  oxygen transfer

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