• 关键词：硒污染  无机硒(亚硒酸钠)  有机硒(硒代蛋氨酸)  土壤酶活性  相对抑制率

• 基金项目：公益性行业（农业）科研专项（No.201303106）；国家自然科学基金（No.41671483）；国家重点基础性科技专项（No.2013FY111100-02）

• 史雅静
• 辽宁科技学院生物医药与化学工程学院, 辽宁省生物医药与化学工程重点实验室, 本溪 117004

• 史雅娟
• 中国科学院生态环境研究中心, 城市与区域国家重点实验室, 北京 100085

• 王玉荣
• 辽宁科技学院生物医药与化学工程学院, 辽宁省生物医药与化学工程重点实验室, 本溪 117004

• 李田春
• 辽宁科技学院生物医药与化学工程学院, 辽宁省生物医药与化学工程重点实验室, 本溪 117004

• 周宝春
• 辽宁科技学院生物医药与化学工程学院, 辽宁省生物医药与化学工程重点实验室, 本溪 117004

• 谢勇
• 辽宁科技学院生物医药与化学工程学院, 辽宁省生物医药与化学工程重点实验室, 本溪 117004

• 徐福友
• 辽宁科技学院生物医药与化学工程学院, 辽宁省生物医药与化学工程重点实验室, 本溪 117004

• 梁若玉
• 中国科学院生态环境研究中心, 城市与区域国家重点实验室, 北京 100085

• 摘要：通过室内非作物连续培养，研究了不同剂量、不同形态外源硒（亚硒酸钠、硒代蛋氨酸）对土壤脲酶、蔗糖酶、中性磷酸酶活性影响的动态变化过程.结果表明，低剂量（10 mg·kg^-1）无机硒（亚硒酸钠）对土壤脲酶和蔗糖酶活性有先激活后抑制作用，对土壤中性磷酸酶活性有激活作用.而相同剂量有机硒（硒代蛋氨酸）处理时，3种酶均表现为不同程度的激活状态.高剂量（>30 mg·kg^-1）无机硒（亚硒酸钠）处理时，3种酶活性均表现先激活后抑制，其中对脲酶影响最大，对蔗糖酶影响次之，对中性磷酸酶影响最小.而施入高剂量（>30 mg·kg^-1）有机硒（硒代蛋氨酸）后，对土壤中脲酶、蔗糖酶的激活作用明显大于无机硒的影响，但对磷酸酶的影响不明显.试验充分说明施入不同剂量、不同形态的外源硒，土壤酶活性表现出不同的动态响应，并且有机硒比无机硒更有利于土壤微生物的生长，能提高土壤酶活性，促进N、P、C养分在土壤生态系统的循环.用有机硒替代无机硒作为外源硒源，有望成为未来发展方向.

• Abstract：The dynamic changes of soil urease, sucrase and neutral phosphatase activity were explored by continuous cultivation without crops in different concentrations and forms of exogenous selenium (sodium selenite and selenium methionine).The results showed that enzyme activities of soil urease and invertase were activated and then inhibited when the exposure concentration of sodium selenite was 10 mg·kg^-1.Under the same conditions the enzyme activities of soil neutral phosphate was activated. When the same concentration of organic selenium (selenomethionine) was processed, the three kinds of enzyme activities of soil showed different degrees of activations. After adding high concentration (>30 mg·kg^-1) of inorganic selenium (sodium selenate),all of the three kinds of enzyme activities in soil were activated and then inhibited, which had the greatest influence on urease, followed by sucrase, which/that had the least effect on neutral phosphatase. After adding high concentration (>30 mg·kg^-1) of organic selenium (selenomethionine), its effect on urease and sucrose enzyme in soil was significantly greater than that of inorganic selenium, but its effect on phosphatase was not obvious. The results fully showed that adding different forms of exogenous selenium had different impacts on soil enzyme activities. The results also showed that organic selenium was more advantageous than inorganic selenium on soil microbial growth, soil enzyme activity and N、P、C nutrient cycling in soil ecosystem. Organic selenium instead of inorganic selenium as the source of selenium might gain more grounds in the future.

• Key words：Se pollution  inorganic selenium (sodium selenite)  organic selenium (selenomethionine)  enzyme activities of soil  relative inhibitory rate

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