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研究报告

Improvement of hydrogen production from Arthrospira maxima through autofermentation by adding Methanothrix concilii

摘要：采用只消耗乙酸盐而不消耗氢气的甲烷丝菌与螺旋藻混合培养，以提高螺旋藻利用自身多糖在自身氢酶作用下暗发酵产氢量.通过培养基调控提高了螺旋藻生长富集的总糖产量，当NaCl浓度由239 mmol·L^-1增加到739 mmol·L^-1，螺旋藻总糖产量提高了107.7%，达到0.54 g·L^-1.螺旋藻在黑暗厌氧条件下加入甲烷丝菌混养后的自发酵产氢量提高了33.8%，达到43.8 mL·g^-1；液相主要代谢产物乙酸盐则提高了69.2%，达到1639.1 μmol·g^-1.同时，螺旋藻加入甲烷丝菌混养后自发酵过程的能量转换效率由6.7%提高到11.6%.甲烷丝菌通过消耗乙酸盐促进了螺旋藻自发酵产氢的乙酸途径反应正向进行，从而提高了氢气产量和能量转换效率.

Abstract：The effect of Methanothrix concilii, which consumes acetate as its lone energy source, on hydrogen production from Arthrospira (Spirulina) maxima through autofermentation was investigated for the first time. During A. maxima cultivation, the effect of different NO₃^- and Na⁺ concentrations on cell growth and the accumulation of carbohydrates was also explored. At a Na⁺ concentration of 739 mmol·L^-1, the total carbohydrate yield increased by 107.7% to 0.54 g·L^-1. The harvested A. maxima suspension was treated under dark and anaerobic conditions to produce hydrogen in autofermentation, with M. concilii added during the fermentation. By adding M. concilii, the hydrogen yield reached up to 43.8 mL·g^-1, an increase of 33.8%. Furthermore, the acetate, as the predominant soluble metabolite product, increased by 69.2% to 1639.1 μmol·g^-1, whilst the energy conversion efficiency was enhanced from 6.7% to 11.6%. By consuming acetate, M. concilii facilitated the acetate metabolic pathway in the autofermentation of A. maxima, resulting in the enhanced hydrogen yield and significantly increased energy conversion efficiency.

Key words：Methanothrix concilii Arthrospira(Spirulina)maxima hydrogen production autofermentation soluble metabolite products