• 关键词：芦苇  UV-B辐射  色素含量  叶绿素荧光

• 基金项目：甘肃省重点研发计划项目（No.18YF1FA078，1504FKCA003）

• 褚润
• 甘肃农业大学资源与环境学院, 兰州 730070

• 陈年来
• 甘肃农业大学资源与环境学院, 兰州 730070

• 摘要：叶绿素荧光是光合作用的探针，可反映光合机构内一系列重要的调节过程.本文在露天条件下研究了不同UV-B辐照强度（对照：168 μW·cm²，低强度：210 μW·cm²，高强度：252 μW·cm²）对芦苇色素含量及叶绿素荧光参数的影响，探讨了芦苇光合生理对UV-B辐射的响应机制.结果表明：与自然光照相比，UV-B辐射增强对芦苇叶片表观造成一定的损伤，且随辐射梯度增大，损伤加剧.与自然光照相比，低辐射初期可促进芦苇叶片叶绿素a、叶绿素b、总叶绿素及类胡萝卜素含量增大，但随辐射时间延长抑制作用显现；高辐射下，芦苇叶绿素a、叶绿素b、总叶绿素及类胡萝卜素含量均显著降低.与自然光照相比，低辐射前期，叶绿素荧光参数无显著变化，随辐照时间延长，F_o、NPQ增高，F_m、F_v/F_m与F_v/F_o显著降低，ΦPSⅡ值、qP降低.高辐射可显著增大F_o、NPQ，显著降低F_m、F_v/F_m、F_v/F_o、ΦPSⅡ，降低qP.试验结束时，处理组NPQ均低于对照，且随辐射梯度增大，下降幅度加剧.UV-B辐射对PSⅡ的破坏作用随辐射强度增大而增大.

• Abstract：Chlorophyll fluorescence is a probe of photosynthesis, which can reflect a series of important regulatory processes in the photosynthetic mechanism. The effects of different intensities UV-B radiation (control:168 μW·cm^-2, low-intensity:210 μW·cm^-2, high-intensity:252 μW·cm^-2) on pigments content and chlorophyll fluorescence of Phragmites australis had been studied in the open air, in order to discuss the response mechanism of photosynthetic physiology to UV-B radiation. The results showed that:1) Compared with the control, enhanced UV-B radiation caused a certain damage to the leaf appearance, with the increase of radiation intensity, the apparent damage degree became more seriously. 2) Compared with natural light, low radiation intensity increased the content of chlorophyll a, chlorophyll b, total chlorophyll and carotenoid. However, the inhibitory effect of the radiation was increased with the prolonged radiation time. High radiation intensity reduced significantly the content chlorophyll a, chlorophyll b, total chlorophyll and carotenoid. 3) Compared with natural light, There were no significant changes in chlorophyll fluorescence parameters at the early stage of low radiation. As the irradiation continues, low radiation intensity increased F_o and NPQ, but reduced the ΦPSⅡ, qP and significantly reduced the F_m, F_v/F_m and F_v/F_o. High radiation intensity significantly increased F_o and NPQ, however reduced qP and significantly reduced F_m, F_v/F_m, F_v/F_o and ΦPSⅡ. At the end of the experiment, NPQ was lower than the control group, and with the increasing radiation intensity, the degree increased. The damage of UV-B radiation to PSⅡ increased with the increased radiation intensity.

• Key words：Phragmites australis  UV-B radiation  pigments content  chlorophyll fluorescence

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