• 关键词：管道施工扰动  高通量测序  土壤养分  细菌多样性  相关性

• 基金项目：中国石油天然气股份有限公司天然气与管道分公司科研项目（No.2014D-4610-0501）

• 芮文鸿
• 兰州理工大学石油化工学院, 兰州 730050

• 陈吉祥
• 兰州理工大学石油化工学院, 兰州 730050

• 王永刚
• 兰州理工大学石油化工学院, 兰州 730050

• 何学良
• 中石油北京天然气管道有限公司, 北京 100101

• 张彦
• 中石油北京天然气管道有限公司, 北京 100101

• 周永涛
• 中石油北京天然气管道有限公司, 北京 100101

• 李彦林
• 兰州理工大学石油化工学院, 兰州 730050

• 张继义
• 兰州理工大学石油化工学院, 兰州 730050

• 摘要：为探索天然气管道施工后土壤植被恢复的影响因素，研究了榆林脆弱黄土区管道施工扰动和周边天然植被区的植被特点、土壤理化性质、细菌群落结构.结果表明：管道施工扰动后，与天然植被区比较，扰动区植被盖度从60%下降到30%，土壤养分除全钾和全磷含量分别増加了29.97%和18.24%外，土壤含水率、有机质、全氮、速效钾、速效磷、速效氮、过氧化氢酶、脲酶、蔗糖酶均显著降低，土壤pH变化明显；扰动区土壤细菌丰度和多样性显著低于天然植被区.变形菌门（Proteobacteria）、酸杆菌门（Acidobacteria）和放线菌门（Actinobacteria）为共同优势细菌，但扰动区变形菌门的丰度显著增加，从30.83%增加到49.26%.在属水平上，假单胞菌属（Pseudomonas）、鞘氨醇单胞菌属（Sphingosine）为共同优势菌属，扰动区两者丰度分别从6.31%和5.28%增加到19.95%和9.69%（p<0.01），表明扰动后逆境适应性强的细菌丰度增加.细菌优势菌属与土壤理化因子相关性分析表明，假单胞菌属分布与土壤理化因子无显著相关性，芽单胞菌属（Gemmatimonas）和鞘氨醇单胞菌属与全钾呈显著负相关（p<0.01），GP6与速效氮呈显著负相关，与速效磷呈显著负相关（p<0.01），与全磷呈显著正相关（p<0.05），表明土壤养分会影响细菌群落结构和多样性.

• Abstract：To explore the restrictive factors for the natural vegetation recovery of the disturbed area caused by gas pipeline construction, the characteristics of vegetation, soil physical chemical properties and bacterial community structure were investigated between the disturbed zone of pipeline construction and the nature vegetation zone in fragile Loess Region, Yulin City, Shaanxi Province. ① The vegetation cover was decreased from 60% to 30% in the disturbed area when compared with that of the nature vegetation zone. The soil pH did not show obvious change. The total contents of phosphorus and potassium of the disturbed soil were increased by 29.97% and 18.24% respectively, and the contents of other major elements such as water, organic matter, total nitrogen, available potassium, available phosphorus, available nitrogen were obviously decreased. The soil enzyme activities such as catalase, urease and socrase were obviously decreased. ② The richness and diversity of bacteria in disturbance soil were obviously lower than those of the natural vegetation soil. Proteobacteria,Acidobacteria and Actinobacteria were the dominant bacteria phyla in the disturbed and natural vegetation soils. However,the abundance of Proteobacteria was apparently increased from 30.83% to 49.26% in the disturbed soil. At the genus level, Pseudomonas and Sphingosine were commonly domain bacteria. The abundances of Pseudomonas and Sphingosine in the natural vegetation areas were only 6.31% and 5.28% respectively, but the abundances of Pseudomonas and Sphingosine were increased to 19.95% and 9.69% in the disturbed soils, which suggested that the abundance of resistant bacteria increased after the disturbance. ③ The correlation analysis between dominant the bacteria genera and soil physicochemical factors showed that the physical and chemical properties of soil had little effects on Pseudomonas distribution (p>0.05), the content of total potassium has a significant negative correlation with Sphingomonas and Gemmatimonas (p<0.01). Significant negative relationships between GP6 and the contents of available nitrogen and phosphorus were also observed (p<0.01). There was a significant positive relationship between GP6 and total phosphorus (p<0.05),which indicated that the structure and diversities of bacteria community were significantly affected by the soil nutrients.

• Key words：pipeline construction disturbance  high throughput sequencing  soil nutrient  bacteria diversity  correlation analysis

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