• 关键词：硝化污泥  提取方法  LB-EPS  TB-EPS  三维荧光光谱

• 基金项目：陕西省教育厅重点科研计划项目（No.17JS079）；西安建筑科技大学青年科技基金（No.QN1715）

• 肖倩
• 西安建筑科技大学 西北水资源与环境生态教育部重点实验室, 西安 710055

• 杨垒
• 西安建筑科技大学 西北水资源与环境生态教育部重点实验室, 西安 710055

• 任勇翔
• 西安建筑科技大学 西北水资源与环境生态教育部重点实验室, 西安 710055

• 汪旭晖
• 西安建筑科技大学 西北水资源与环境生态教育部重点实验室, 西安 710055

• 朱鹏涛
• 西安建筑科技大学 西北水资源与环境生态教育部重点实验室, 西安 710055

• 陈宁
• 西安建筑科技大学 西北水资源与环境生态教育部重点实验室, 西安 710055

• 摘要：采用温和加热法、超声法、超声+振荡法和离心法4种方法提取硝化污泥松散结合型胞外聚合物（LB-EPS），及乙二胺四乙酸（EDTA）法、甲醛+NaOH法、甲醛+超声、阳离子树脂法（CER）和加热法提取硝化污泥紧密结合型胞外聚合物（TB-EPS），通过化学分析结合三维荧光（3D-EEM）光谱对各提取方法进行比较.结果表明，LB-EPS和TB-EPS的最优提取方法分别为温和加热法和甲醛+NaOH法，TOC提取量分别为16.82、58.43 mg·g^-1.三维荧光光谱显示温和加热法能有效提取LB-EPS中的类色氨酸和类腐殖酸；甲醛+NaOH法、CER和加热法提取的TB-EPS中物质最为全面，主要包括类色氨酸、类腐殖酸和类酪氨酸.TB-EPS三维荧光浓度效应分析表明荧光峰位置与EPS浓度变化无关；类腐殖酸和类酪氨酸荧光峰强度随EPS浓度降低而减小，分别呈二项式和对数关系.

• Abstract：Four methods including temperate heating, ultrasonication, ultrasonication + oscillation and centrifugation were employed for extracting loosely bound extracellular polymeric substances (LB-EPS), and the ethylenediamine tetraacetic acid (EDTA), formaldehyde + NaOH, formaldehyde + ultrasonication, cation exchange resin (CER) and heating methods were used to extract tightly bound extracellular polymeric substances (TB-EPS) of nitrifying sludge. The extraction methods were evaluated by chemical analysis and three-dimensional excitation-emission matrix (3D-EEM) fluorescence spectroscopy. The experimental results revealed that temperate heating and formaldehyde + NaOH were the optimal extraction conditions respective for LB-EPS and TB-EPS, and the maximum values of TOC were 16.82 and 58.43 mg·g^-1, respectively. The 3D-EEM fluorescence spectroscopy showed tryptophan-like and humic acid-like substances in LB-EPS could be effectively extracted by temperate heating. The formaldehyde + NaOH, CER and heating could extract the most comprehensive substances in TB-EPS, including tryptophan-like, humic acid-like and tyrosine-like substances. The effect of EPS concentration on 3D-EEM fluorescence spectra indicated that fluorescence peak position was not related to the change of EPS concentration. The fluorescence intensity of the humic acid-like and tyrosine-like in TB-EPS decreased with the decreasing of EPS concentration, and their functional relationships were binomial and logarithmic, respectively.

• Key words：nitrifying sludge  extraction method  LB-EPS  TB-EPS  3D-EEM

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