研究报告
牛珠玉,陈元彩,胡勇有.恶臭假单胞菌原位还原Pd(0) NPs对微生物电子传递性能及活性的影响[J].环境科学学报,2018,38(6):2320-2326
恶臭假单胞菌原位还原Pd(0) NPs对微生物电子传递性能及活性的影响
- The study about the influence of P. putida-reduced Pd(0) NPs toward bacteria electron transfer and bacteria activity
- 基金项目：国家自然科学基金（No.21677052）；广东省水利科技创新项目（No.2017-25）；产学研重大创新项目（No.201704020206，2016B090918104）
- 作者
- 单位
- 牛珠玉
- 华南理工大学环境与能源学院, 工业聚集区污染控制与生态修复教育部重点实验室, 广州 510006
- 陈元彩
- 华南理工大学环境与能源学院, 工业聚集区污染控制与生态修复教育部重点实验室, 广州 510006
- 胡勇有
- 华南理工大学环境与能源学院, 工业聚集区污染控制与生态修复教育部重点实验室, 广州 510006
- 摘要：微生物原位合成零价金属纳米颗粒在材料合成和环境应用领域引起了广泛关注和研究.本研究利用Pseudomonas putida（P. putida）合成Pd（0）纳米颗粒（NPs），通过FESEM、TEM、XPS等对合成的纳米颗粒进行形貌、价态表征，并对其电化学性能及微生物抑制作用进行分析.结果表明，在厌氧条件下，P. putida在胞外及细胞周质空间可原位合成粒径约10 nm的Pd（0） NPs.CV实验表明，相比P. putida，含Pd（0） NPs的P. putida有更高的氧化还原电流；随着初始反应Pd（Ⅱ）浓度增加至0.07和0.1 mmol·L-1，恒电位实验中的乳酸氧化电流分别升至（10.6±5.2）和（22.5±9.7） nA，分别为P. putida（（5.7±2.8） nA）的1.85和3.95倍；TTC-ETS和INT-ETS分别提升至89.02和209.09 μg·mg-1·h-1，分别是P. putida的1.64和1.34倍.以上结果证明了Pd（0） NPs对微生物胞外电子传递（Extracellular Electron Transfer，EET）的正面影响.抑菌圈实验和激光共聚焦图像表明，低量Pd（0） NPs具有较好的生物相容性，过多Pd（Ⅱ）和Pd（0） NPs对微生物有一定的毒性.
- Abstract：In-situ synthesis of zero valent metal nanoparticles(NPs) by microbes has attracted ample attentions in the field of metal materials synthesis. In this study, bio-reduced Pd(0) NPs were obtained by a gram-negative bacterium, Pseudomonas putida(P. putida), and their effects on P. putida were also discussed. The FESEM, TEM and XPS results showed that the bio-reduced Pd(0) NPs(~10 nm) were evenly distributed in the surface and periplasm of P. putida cells. In addition, CV experiments suggested that bio-reduced Pd(0) NPs evidently enhanced the electrochemical performance of P. putida. In potentiostatic results, with an increase of initial Pd(Ⅱ) concentrations(0.07 and 0.1 mmol·L-1), the lactate-oxidation current increased to (10.6±5.2) and (22.5±9.7) nA, respectively, which were 1.85 and 3.95 times larger than those of pristine P. putida((5.7±2.8) nA). Similarly, TTC-ETS and INT-ETS also increased to 89.02 and 209.09 μg·mg-1·h-1, which were 1.64 and 1.34 times larger than those of pristine P. putida. Furthermore, the bacteriostatic circle experiment and CSLM images indicated that low levels of Pd(Ⅱ) and Pd(0) NPs were compatible with P. putida, and high levels of Pd(Ⅱ) and Pd(0) NPs showed toxicity to P. putida. All the above results demonstrated that the bio-reduced Pd(0) NPs exhibited positive improvement on microbial extracellular electron transfer(EET), but their concentrations should be severely considered.
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