研究报告
任杰辉,程文,万甜,王敏,张晓晗.缓冲液盐度对热提取活性污泥胞外聚合物的影响[J].环境科学学报,2018,38(8):3054-3060
缓冲液盐度对热提取活性污泥胞外聚合物的影响
- Effect of buffer salinity on heat extraction of extracellular polymeric substances from activated sludge
- 基金项目：国家自然科学基金（No.51679192，51709224）；陕西重点研究开发项目（No.2017SF-392）
- 作者
- 单位
- 任杰辉
- 西安理工大学水利水电学院, 西北旱区生态水利国家重点实验室, 西安 710048
- 程文
- 西安理工大学水利水电学院, 西北旱区生态水利国家重点实验室, 西安 710048
- 万甜
- 西安理工大学水利水电学院, 西北旱区生态水利国家重点实验室, 西安 710048
- 王敏
- 西安理工大学水利水电学院, 西北旱区生态水利国家重点实验室, 西安 710048
- 张晓晗
- 西安理工大学水利水电学院, 西北旱区生态水利国家重点实验室, 西安 710048
- 摘要：采用热提取法提取污泥中微生物细胞外的胞外聚合物（Extracellular Polymeric Substance，EPS），分析了不同提取时间、提取温度、缓冲液盐度对污泥中EPS提取效果的影响，并采用平行因子模型（PARAFAC模型）分析了不同缓冲液盐度下EPS的荧光组分及特性，探究了缓冲液盐度对热提取污泥EPS的影响.结果表明，缓冲液盐度对热提取EPS的组分和荧光物质的强度均产生影响，0.50%的氯化钠缓冲液盐度条件下松散型EPS（loosely bound EPS，LB-EPS）组分含量及荧光物质最佳，0.05%氯化钠缓冲液盐度条件下紧密型EPS（tightly bound EPS，TB-EPS）的提取效果最佳；当提取时间为40 min，提取温度为70℃时，总EPS含量达（93.26±2.07） mg·g-1（以VSS计）；三维荧光结果显示，不同缓冲液盐度条件下污泥中EPS均包含类蛋白质（C1：λEx/λEm=290 nm，302/350 nm）、类色氨酸（C2：λEx/λEm=278 nm，340 nm）、类胡敏酸（C3：λEx/λEm=310 nm，410 nm）和类腐殖酸（C4：λEx/λEm=380/420 nm，474/514 nm）4类荧光组分峰，在溶解性EPS（SMP）和LB-EPS中类色氨酸的荧光强度最高，TB-EPS中类蛋白质荧光强度最高.因此，合适的热提取缓冲液盐度，有利于污泥EPS中各组分的高效提取.
- Abstract：In this paper, the heat extraction method was used for extracting extracellular polymeric substances (EPS) from microbial cells in activated sludge. The effects of extraction time, extraction temperature and buffer salinity on the EPS extraction were analyzed. The parallel factor model (PARAFAC model) was used to study the fluorescence components and properties of EPS in different buffer salinities. The result showed that buffer salinity greatly affected the substance content and the intensity of fluorescent substance of heat extracting EPS. For the content of loosely bound EPS (LB-EPS) was high and the intensity of fluorescent substance was heavy with a sodium chloride buffer salinity of 0.50%; and for tightly bound EPS (TB-EPS), extraction the sodium chloride buffer salinity of 0.05% has the best efficiency. The total extracting content of EPS reached to (93.26±2.07) mg·g-1 with a temperature of 70℃ and extraction time of 40 min. The results of three-dimensional fluorescence analysis showed that EPS in activated sludge contained four kinds of fluorescent component peaks of protein-like (C1:λEx/λEm=290 nm, 302/350 nm), tryptophan-like (C2:λEx/λEm=278 nm, 340 nm), humilic acid-like (C3:λEx/λEm=310 nm, 410 nm) and humic acids-like (C4:λEx/λEm=380/420 nm, 474/514 nm) in different buffer salinities. Tryptophan-like fluorescent intensity was the highest in soluble EPS (SMP) and LB-EPS, and protein-like fluorescent intensity was the highest in TB-EPS. Therefore, an appropriate buffer salinity of heat extraction was beneficial to the extraction of the components in sludge EPS.
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