• 关键词：多氯联苯  玉米  丛枝菌根真菌  猪炭  细菌群落结构

• 基金项目：浙江省科技厅公益性项目（No.2016C33030）；国家大学生创新训练项目（No.20132000052）

• 王楚栋
• 1. 浙江省土壤污染生物修复重点实验室, 杭州 311300;2. 浙江农林大学环境与资源学院, 杭州 311300

• 单明娟
• 1. 浙江省土壤污染生物修复重点实验室, 杭州 311300;2. 浙江农林大学环境与资源学院, 杭州 311300

• 陆扣萍
• 1. 浙江省土壤污染生物修复重点实验室, 杭州 311300;2. 浙江农林大学环境与资源学院, 杭州 311300

• 王海龙
• 1. 浙江省土壤污染生物修复重点实验室, 杭州 311300;2. 佛山科学技术学院环境与化学工程学院, 佛山 528000

• 赵未央
• 浙江农林大学环境与资源学院, 杭州 311300

• 黄圣妍
• 浙江农林大学环境与资源学院, 杭州 311300

• 余樾
• 浙江农林大学环境与资源学院, 杭州 311300

• 吾尔开西·买木里
• 浙江农林大学环境与资源学院, 杭州 311300

• 秦华
• 1. 浙江省土壤污染生物修复重点实验室, 杭州 311300;2. 浙江农林大学环境与资源学院, 杭州 311300

• 摘要：选用玉米（Zea mays L.）为供试植物，采用盆栽试验研究了接种丛枝菌根（Arbuscular mycorrhiza，AM）真菌（Funneliformis mosseae）和添加不同粒径猪炭对多氯联苯（Polychlorinated biphenyls，PCBs）污染土壤的联合修复效应及其对土壤微生物的影响.结果表明，接种AM真菌（10%接种量）及添加2.5%猪炭对土壤有效磷含量提高具有显著的协同效应，猪炭还显著提高了土壤有机碳、速效钾含量和pH值（p<0.05）；猪炭显著促进了菌根真菌侵染率，但对玉米根系生物量具有抑制作用.接种AM真菌的同时添加猪炭提高了细菌16S rDNA丰度，且接种AM真菌同时添加粒径>0.25 mm猪炭显著促进了土壤PCBs降解率.AM真菌与猪炭改变了土壤微生物种群的相对丰度，其中，Planctomycetes与土壤三氯联苯降解显著相关（r=0.049，p<0.05），而Acidobacteria与五氯联苯降解显著相关（r=0.008，p<0.01）.AM真菌及猪炭提高了土壤有效养分含量，促进了植物生物量和土壤PCBs降解，对PCBs污染土壤具有较好的修复潜力.

• Abstract：The combined effects of arbuscular mycorrhizal (AM) fungi (Funneliformis mosseae) and dead pig biochar with two particle sizes on soil microbial community and PCBs dissipation in a contaminated soil planted with Zea mays L. was studied using a pot experiment. The results showed that 10% inoculation of AM fungi and 2.5% dead pig biochar amendment had significant synergistic effect on the increase of soil available P concentration (p<0.05). Amendment of dead pig biochar also significantly increased soil pH and concentrations of soil organic carbon and available K (p<0.05). Dead pig biochar significantly increased root mycorrhizal colonization rate significantly, but decreased the root biomass of Zea mays L. (p<0.05). AM fungi inoculation combined with dead pig biochar increased soil bacterial abundance, among which biochar with >0.25 mm particle size biochar and AM fungi had significantly synergistic effect on soil PCBs dissipation. AM fungi and biochar amendment changed the relative abundance of bacterial taxa, among which Planctomycetes and Acidobacteria had significant correlation with soil tri-(r=0.049, p<0.05) and penta-CBs (r=0.008, p<0.01) dissipation, respectively. In conclusion, AM fungi and dead pig biochar increased soil nutrient availability, plant biomass and soil PCBs dissipation rate, indicating that they have great potential in the remediation of PCBs contaminated soils.

• Key words：polychlorinated biphneyls  Zea mays L.  arbuscular mycorrhizal fungi  dead pig biochar  bacterial community composition

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