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赵娜娜,许继飞,宋晓雪,田鹏,丁舒心,赵吉.嗜盐高效降酚菌株Halomonas sp.H17的筛选及降解苯酚特性[J].环境科学学报,2019,39(2):318-324

Screening and phenol-degrading characteristics of a highly efficient phenol-degrading halophilic bacterial strain Halomonas sp. H17

摘要：从巴丹吉林沙漠盐湖沉积物中分离获得1株在高盐环境下高效降解苯酚菌H17.分析了H17生理生化特性、16S rDNA基因序列、苯酚降解特性及动力学，结果表明，H17属于盐单胞菌属（Halomonas sp.），能在0~20%的盐度下有效降解苯酚，每升外加适量的碳源（葡萄糖浓度0.8 g）和复合氮源（KNO₃ 1 g、NH₄Cl 5 g、酵母提取物0.2 g和胰蛋白胨0.2 g）能够促进H17的生长及降解苯酚能力.在温度为30℃、pH 7~8、盐度5~10%的条件下，H17均能高效降解苯酚，最高降解率可达到88.5%.该菌株降解苯酚动力学符合Halane模型，经拟合其生长参数为μ_max=0.31 h^-1，K_S=191.63 mg·L^-1，K_i=683.05 mg·L^-1.研究显示H17具有在高盐环境下降解和耐受苯酚的能力，同时对环境有较强的适应性，体现出其在高盐含酚废水实际处理中具有良好的应用价值.

Abstract：A highly efficient phenol-degrading bacterial strain was separated from the sediments of Badain Jaran Desert Salt Lake in the hypersaline condition. The physiological and biochemical characteristics, 16S rDNA gene sequences, and phenol-degrading kinetics were analyzed. The results revealed that the bacterial strain H17 belonged to Halomonas sp., which could efficiently degrade phenol under 0~20% salinity. Furthermore, adding appropriate carbon source (0.8g glucose) and nitrogen sources (i.e. 1 g KNO₃, 5 g NH₄Cl, 0.2 g yeast extract, and 0.2 g tryptone) could promote the growth of H17 as well as phenol degradation. Under optimal degradation conditions (30℃, pH 7~8, salinity 5%~10%), the highest degradation rate of phenol by H17 could reach up to 88.5%. The phenol-degrading kinetics of this bacterial strain was characterized using Halane-model, with the growth parameters of μ_max=0.31 h^-1,K_S=191.63 mg·L^-1,K_i=683.05 mg·L^-1 according to the over-fitting. This study showed that the bacterial strain H17 could tolerate and degrade phenol in the hyperhaline environment, suggestive of its application value for hyperhaline phenolic wastewater treatments.