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段慧玲,夏瑾,梁刚,秦国华,桑楠.PM2.5对小鼠和H9C2细胞心肌肥大相关因子mRNA表达的影响[J].环境科学学报,2019,39(3):978-984
PM2.5对小鼠和H9C2细胞心肌肥大相关因子mRNA表达的影响
- Effect of PM2.5 on mRNA expression of cardiac hypertrophy-related factors in mice and H9C2 cells
- 基金项目：国家自然科学基金（No.21777091）；广州市环境暴露与健康重点实验室开放基金（No.GZKLEEH201612）；环境化学与生态毒理学国家重点实验室开放基金（No.KF2016-17）
- 作者
- 单位
- 段慧玲
- 山西大学环境与资源学院, 太原 030006
- 夏瑾
- 山西大学环境与资源学院, 太原 030006
- 梁刚
- 山西大学环境与资源学院, 太原 030006
- 秦国华
- 1. 山西大学环境与资源学院, 太原 030006;2. 暨南大学环境学院, 广州市环境暴露与健康重点实验室, 广州 510632
- 桑楠
- 山西大学环境与资源学院, 太原 030006
- 摘要：用采自太原市4个不同季节的细颗粒物（PM2.5）对小鼠心肌细胞H9C2进行染毒后，采用荧光定量PCR技术检测心肌肥大相关因子ANP和TGF-β的mRNA水平.采用浓度分别为0、1、3、10 μg·mL-1的冬季PM2.5处理H9C2细胞后，检测心肌肥大相关因子ANP、β-MHC、MMP2和MMP9的mRNA水平.之后分别选取4周龄、4月龄、10月龄C57BL/6雌性小鼠作为实验模型，采用咽后壁滴注的方法用3 mg·kg-1 PM2.5暴露4周.采用苏木素-伊红染色（H&E）对小鼠心脏组织病理切片进行观察，并检测ANP和β-MHC的mRNA水平.结果发现，与对照组相比，冬季PM2.5诱导H9C2细胞中ANP和TGF-β的mRNA水平升高最为显著；其中，当冬季PM2.5染毒浓度较高时可诱导ANP、β-MHC、MMP2和MMP9的mRNA水平均显著升高.经PM2.5暴露4周后，幼年和老年小鼠心肌细胞核质比显著降低.老年小鼠心脏组织中ANP和β-MHC的mRNA表达水平显著上升.实验表明，冬季PM2.5诱导心肌肥大标志物表达改变的效应要强于其他季节，且有一定的剂量效应关系，而老年小鼠对PM2.5诱导的心肌肥大效应最为敏感.
- Abstract：H9C2 cells were exposed to fine particulate matters (PM2.5) from four different seasons in Taiyuan City, the mRNA levels of ANP and TGF-β related to cardiac hypertrophy were detected by real-time PCR. H9C2 cells were exposed to winter PM2.5 at a concentration of 0, 1, 3, and 10 μg·mL-1, the mRNA levels of ANP, β-MHC, MMP2, and MMP9 were measured by real-time PCR. Female C57BL/6 mice at 4-week, 4-month, and 10-month were exposed to 3 mg·kg-1 PM2.5 for 4 weeks by the mesns of posterior pharyngeal wall drip method. The histological sections of heart were observed by hematoxylin-eosin staining (H&E); the mRNA levels of ANP and β-MHC were detected. The results showed that the mRNA levels of ANP and TGF-β in H9C2 cells were significantly increased after exposed to winter PM2.5 compared with the control group. Winter PM at higher concentrations could increase the mRNA levels of ANP, β-MHC, MMP2 and MMP9. After four weeks exposure, the nucleoplasmic ratios of cardiomyocytes in young and older mice were significantly reduced.The mRNA expression levels of ANP and β-MHC in cardiac tissues of older mice were significantly increased. The study indicated that the effect of the winter PM2.5 increased cardiac hypertrophy markers expression is stronger than other seasons,with a dose-effect manner. While older mice are the most sensitive to PM2.5-induced cardiac hypertrophy.
- Key words：PM2.5 mice H9C2 cells cardiac hypertrophy different age
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