吴韵斐,何义亮,袁其懿,杨俊,张波.水源型水库抗生素抗性基因赋存特征研究[J].环境科学学报,2019,39(6):1834-1841
水源型水库抗生素抗性基因赋存特征研究
- Study on antibiotic resistance genes characteristics in a drinking water reservoir
- 基金项目：水体污染控制与治理科技重大专项（No.2017ZX07207002）
- 吴韵斐
- 上海交通大学, 环境科学与工程学院, 上海 200240
- 何义亮
- 上海交通大学, 环境科学与工程学院, 上海 200240
- 袁其懿
- 上海交通大学, 环境科学与工程学院, 上海 200240
- 杨俊
- 上海交通大学, 环境科学与工程学院, 上海 200240
- 张波
- 上海交通大学, 环境科学与工程学院, 上海 200240
- 摘要：饮用水水源地中抗生素抗性基因会威胁生态安全和人类健康.为了探究抗生素抗性基因（Antibiotic Resistance Genes，ARGs）在金泽水源型水库中的赋存特征，以及水库净化措施对抗生素抗性基因的净化作用，选择微生物活性较高的夏季时期采集水样，采用高通量荧光定量PCR和16S rRNA实时荧光定量PCR进行研究.结果表明，该水库检出62种ARGs；多重抗药类、氨基糖苷类和磺胺类ARGs为水库内占主导的抗性基因.抗生素抗性基因绝对丰度从水库的预处理区至生态净化区，再至输水区呈逐渐降低的趋势，表明水库净化措施对削减ARGs含量有一定作用.抗性基因丰度与可移动基因元件（Mobile Genetic Elements，MGEs）丰度存在显著相关性（p<0.05），表明MGEs对ARGs的水平转移、传播和富集具有重要作用.
- Abstract：Antibiotic resistance genes (ARGs) in drinking water source may threaten the ecological system and human health. Water samples were collected in summer and then were analyzed using high-throughput quantitative PCR (HT-qPCR) and real-time quantitative PCR (RT-qPCR), in order to investigate the characteristics of ARGs in Jinze drinking water reservoir and the impact of the water purification processes of the reservoir on ARGs. The results show that 62 target ARGs were detected in the reservoir. Multidrug resistance genes, aminoglycoside resistance genes and sulfonamide resistance genes were the dominant ARGs in the reservoir. The absolute abundances of ARGs in the reservoir show a decline trend from the pretreatment area to the ecological purification area and the water delivery area, indicating that the purification processes in the reservoir played a certain role in the attenuation of ARGs. The abundance of ARGs was significantly correlated to the abundance of mobile genetic elements (MGEs) (p<0.05), implicating that MGEs play an important role in the horizontal transfer, dissemination and accumulation of ARGs.
