研究论文
吴康,杨玉蓉,周晨,蒋瑾,司友斌.有机酸强化Fe(III)还原介导的Shewanella oneidensis MR-1降解磺胺甲噁唑[J].环境科学学报,2019,39(7):2117-2124
有机酸强化Fe(III)还原介导的Shewanella oneidensis MR-1降解磺胺甲噁唑
- Organic acids enhanced and Fe(III) reduction mediated sulfamethoxazole degradation by Shewanella oneidensis MR-1
- 基金项目：国家自然科学基金面上项目（No.41471405）
- 作者
- 单位
- 吴康
- 安徽农业大学资源与环境学院, 农田生态保育与污染防控安徽省重点实验室, 合肥 230036
- 杨玉蓉
- 安徽农业大学资源与环境学院, 农田生态保育与污染防控安徽省重点实验室, 合肥 230036
- 周晨
- 安徽农业大学资源与环境学院, 农田生态保育与污染防控安徽省重点实验室, 合肥 230036
- 蒋瑾
- 安徽农业大学资源与环境学院, 农田生态保育与污染防控安徽省重点实验室, 合肥 230036
- 司友斌
- 安徽农业大学资源与环境学院, 农田生态保育与污染防控安徽省重点实验室, 合肥 230036
- 摘要：在实验室条件下，研究了Shewanella oneidensis MR-1对磺胺甲噁唑（Sulfamethoxazole，SMX）的降解特性以及低分子有机酸强化Fe（Ⅲ）还原介导的S.oneidensis MR-1对SMX的降解.结果表明，S.oneidensis MR-1对不同浓度SMX的降解能力与体系中的有机酸和Fe（Ⅲ）密切相关.低浓度SMX对其生长影响不大，高浓度时细菌生长则受到抑制，进而抑制SMX的降解率.当低分子有机酸单独加入体系后，S.oneidensis MR-1对SMX的最大降解率为58.5%；Fe（Ⅲ）单独加入体系后，S.oneidensis MR-1对SMX的最大降解率为67.6%.此外，当柠檬酸和Fe（OH）3同时加入体系后，S.oneidensis MR-1对SMX生物降解和铁还原作用促进效果最显著，培养5 d后SMX降解率为90.3%.最后，采用液相色谱-质谱联用仪（LC-MS）对SMX的降解中间产物进行了分析，推测SMX的可能降解途径是由S-N键断裂引起的.本研究以期为地下水污染场地修复提供理论依据.
- Abstract：In this paper, low molecular organic acids enhanced and Fe(Ⅲ) reduction mediated SMX degradation by Shewanella oneidensis MR-1 were studied under laboratory conditions. The results indicate that the degradation of SMX at different initial concentrations by S. oneidensis MR-1 was closely related to the organic acids and Fe(Ⅲ) contents in the system. SMX at low concentration had little effect on the strains growth. Cells growth was inhibited at high SMX concentration, thereby reducing the degradation efficiency of SMX. When low molecular organic acids were added to the system alone, the maximum degradation rate of SMX by S. oneidensis MR-1 was 58.5%; When Fe(Ⅲ) were added to the system alone, the maximum degradation rate of SMX by S. oneidensis MR-1 was 67.6%. In addition, when 1.0 mmol·L-1 citric acid and 5.0 mmol·L-1 Fe(OH)3 suspensions were simultaneously added to the system, the SMX degradation rate were 90.3%. Finally, the degradation intermediate products of SMX by S. oneidensis MR-1 were analyzed by liquid chromatography-mass spectrometry (LC-MS), and the possible degradation pathway of SMX was caused by S-N bond cleavage. This research provides a theoretical basis and technical support for the remediation of contaminated groundwater.
- Key words：shewanella oneidensis MR-1 sulfamethoxazole biodegradation low molecular organic acids iron reduction
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