张倩,吕红,周杨,王晓磊,张宝瑞.Pseudomonas sp.HS-2厌氧转化双酚F及疏水性蒽醌类化合物的促进作用[J].环境科学学报,2019,39(9):2938-2944
Pseudomonas sp.HS-2厌氧转化双酚F及疏水性蒽醌类化合物的促进作用
- Anaerobic biotransformation of bisphenol F by Pseudomonas sp. HS-2 and promoted by hydrophobic anthraquinone compounds
- 基金项目：国家自然科学基金（No.21577015）
- 张倩
- 大连理工大学工业生态与环境工程教育部重点实验室, 大连 116024
- 吕红
- 大连理工大学工业生态与环境工程教育部重点实验室, 大连 116024
- 周杨
- 大连理工大学工业生态与环境工程教育部重点实验室, 大连 116024
- 王晓磊
- 大连理工大学工业生态与环境工程教育部重点实验室, 大连 116024
- 张宝瑞
- 大连理工大学工业生态与环境工程教育部重点实验室, 大连 116024
- 摘要：为揭示双酚F的厌氧归趋及提高双酚F的厌氧生物转化速率，本文以硝酸盐为电子受体，研究了Pseudomonas sp.HS-2厌氧转化双酚F的特性，并外加4种疏水性蒽醌类化合物（蒽醌、1-氨基蒽醌、2-氨基蒽醌、2-羟基蒽醌）来促进双酚F的厌氧转化.结果表明，菌株HS-2能够将双酚F厌氧转化为4，4-二羟基二苯基甲酮.最适培养条件为：硝酸盐5 mmol·L-1、pH=7和温度35℃.在最适条件下，4种疏水性蒽醌类化合物均能促进双酚F厌氧转化，且2-氨基蒽醌的促进作用最明显.当2-氨基蒽醌浓度为20 mg·L-1时，准一级反应速率常数为2.9×10-2 h-1，较不加2-氨基蒽醌体系提高了37.1%.在无硝酸盐存在时，2-氨基蒽醌不能使菌株HS-2厌氧转化双酚F.但当硝酸盐存在时，外加2-氨基蒽醌则能够促进双酚F的厌氧转化.
- Abstract：To reveal the fate and promote bisphenol F anaerobic biotransformation, the characteristics of bisphenol F biotransformation by Pseudomonas sp. HS-2 were studied using nitrate as an electron acceptor. Four hydrophobic anthraquinone compounds (ACs) anthraquinone, 1-aminoanthraquinone, 2-aminoanthraquinone (2-AAQ), 2-hydroxyanthraquinone were added to promote the biotransformation. The results show that strain HS-2 could anaerobically transform bisphenol F to 4,4-dihydroxybenzophenone. The optimal reaction conditions were 5 mmol·L-1 nitrate, pH 7 and 35 ℃. All the four hydrophobic ACs could promote the biotransformation, 2-AAQ has the highest efficiency. Under 20 mg·L-1 of 2-AAQ, the pseudo-first-order reaction rate constant reached 2.9×10-2 h-1, 37.1% higher than that without 2-AAQ.
