崔晓莹,管博,李正炎.雌二醇、壬基酚和三丁基锡对草金鱼血浆卵黄蛋白原含量的联合效应[J].环境科学学报,2019,39(9):3180-3187
雌二醇、壬基酚和三丁基锡对草金鱼血浆卵黄蛋白原含量的联合效应
- The combined effects of estradiol, nonylphenol and tributyltin on plasma vitellogenin in goldfish Carassius auratus
- 基金项目：国家自然科学基金（No.41476090）
- 崔晓莹
- 中国海洋大学环境科学与工程学院, 青岛 266100
- 管博
- 中国海洋大学环境科学与工程学院, 青岛 266100
- 李正炎
- 1. 中国海洋大学环境科学与工程学院, 青岛 266100;2. 中国海洋大学海洋环境与生态教育部重点实验室, 青岛 266100
- 摘要：雌二醇（17β-estradiol，E2）是生物合成的天然雌激素，壬基酚（nonylphenol，NP）和三丁基锡（tributyltin，TBT）则是人工合成的环境拟雌激素和环境抗雌激素，这3种污染物在自然水体中均广泛分布，给多种水生生物的健康造成威胁，然而其共同存在下的联合效应尚不明确.本文以草金鱼（Carassius auratus）为受试生物，以鱼体血浆中卵黄蛋白原浓度为观测指标，研究雌二醇和壬基酚的雌激素活性及三丁基锡的抗雌激素活性.在单独暴露实验的基础上，研究3种污染物两两联合及三者联合条件下对雄性草金鱼卵黄蛋白原的联合诱导效应.结果表明，雌二醇和壬基酚均能显著诱导雄性草金鱼合成卵黄蛋白原，三丁基锡对雄性和雌性草金鱼卵黄蛋白原含量均无显著影响.雌二醇和壬基酚共暴露时，壬基酚能促进雌二醇的雌激素效应；雌二醇与三丁基锡共暴露时，后者可抑制雌二醇的雌激素效应；3种污染物共同暴露时，三丁基锡也表现为抗雌激素效应.但壬基酚与三丁基锡共暴露时，后者未能抑制壬基酚的雌激素效应.上述结果表明天然雌激素、拟雌激素和抗雌激素之间存在复杂的联合效应，拟雌激素和抗雌激素的毒性效应并非简单地相互抵消，因此在生态风险评价时需全面评估不同类型环境雌激素之间的复合效应.
- Abstract：17β-estradiol is a natural estrogen, whereas nonylphenol (NP) and tributyltin (TBT) are synthetic environmental estrogen mimic and antiestrogen, respectively. These three chemicals are widely distributed in natural waters, posing a threat to the health of many aquatic species. However, the combined effects of these chemicals are still unclear. In this study, the estrogenic effects of E2 and NP, and the antiestrogenic effects of TBT were investigated by using plasma vitellogenin (Vtg) induction in male and female goldfish as the endpoint. Based on the results of single exposure experiments, the combined effects of binary and ternary mixtures of E2, NP and TBT were also investigated. The results show that the plasma Vtg could be induced significantly in male goldfish by E2 and NP, but not by TBT in either male or female goldfish. In the combined exposure groups of E2 and NP, the estrogenic effect of E2 could be strengthened by NP. In the combined exposure groups of E2 and TBT, the estrogenic effect of E2 could be weakened by TBT. In the combined exposure groups of ternary mixtures, TBT inhibited the estrogenic effects of E2 and NP as well. However, in the combined exposure groups of NP and TBT, TBT could not inhibit the estrogenic effect of NP in male goldfish. It is supposed that the combined effects of estrogenic chemicals were complicated with the presence of natural estrogen, estrogen mimic and antiestrogen. The effects of estrogen and antiestrogen in combination could not be merely neutralized and comprehensive evaluation of combined effects of various categories of estrogens therefore requires further study for ecological risk assessment in natural environment.
