陈惠鑫,佟娟,陈奕童,程荣,郑祥.再生水补给型城市景观水体中抗生素抗性基因的污染特征——以圆明园为例[J].环境科学学报,2019,39(12):4057-4063
再生水补给型城市景观水体中抗生素抗性基因的污染特征——以圆明园为例
- Pollution characteristics of antibiotic resistance genes in urban landscape water supplied by reclaimed water: Taking Yuanmingyuan as an example
- 基金项目：中央高校建设世界一流大学（学科）和特色发展引导专项资金资助
- 佟娟
- 中国科学院生态环境研究中心, 北京 100085
- 摘要：由于抗生素抗性基因（Antibiotic Resistance Genes，ARGs）对环境和人类健康存在潜在威胁而逐渐受到人们的重视，高人口密度城市中典型景观水体是ARGs储存库和传播媒介.本研究采用实时荧光定量PCR技术，研究了圆明园不同点位的ARGs污染情况和分布特征.结果表明：园区中ARGs的绝对丰度范围在n.d.（未检出）~1.08×109 copies·L-1之间；园区进水口目标ARGs的丰度低于末端，表明封闭的水体可能为ARGs的积累提供了理想培养基.在所检测基因中，磺胺类ARGs占主导，ARGs的绝对丰度依次为：sulII > sulI > mefA > tetQ > tetM > ermB.磺胺类ARGs与指示微生物粪大肠菌群和大肠杆菌间存在显著相关性，表明指示微生物在ARGs的传播中可能起着关键作用.
- Abstract：Antibiotic Resistance Genes (ARGs) are gaining more and more attention because of their potential threat to the environment and human health. The typical landscape water in high population density cities is the medium for the source and spread of ARGs. In this study, quantitative real-time PCR technology was used to study the contamination and distribution of ARGs at in Yuanmingyuan. The results show that the absolute abundance of ARGs in the park ranged from n.d. to 1.08×109 copies·L-1. The abundance of ARGs in the inlet of the park is lower than the end, indicating that the closed water may provide an ideal medium for the accumulation of ARGs. The absolute abundance of ARGs is:sulII > sulI > mefA > tetQ > tetM > ermB. Sulfonamide ARGs predominated among the genes tested in this study and there was a significant correlation between sulfonamide ARGs and the indicated microbial fecal coliforms (FC) and Escherichia coli (E.coli), indicating that the microbes may play a key role in the spread of ARGs.
