施昊坤,吴次芳,张茂鑫,林耀奔,胡一鸣.土地整治对工业区周边土壤微生物多样性和群落结构影响分析[J].环境科学学报,2020,40(1):212-223
土地整治对工业区周边土壤微生物多样性和群落结构影响分析
- Effects of land consolidation on soil microbial diversity and community structure around industrial zone
- 基金项目：国家社会科学基金重大项目（No.14ZDA039）；山东黄河三角洲土地利用和生态工程技术研究中心项目；山东省盐碱地综合开发示范项目
- 施昊坤
- 浙江大学土地与国家发展研究院, 杭州 310058
- 吴次芳
- 浙江大学土地与国家发展研究院, 杭州 310058
- 张茂鑫
- 浙江大学土地与国家发展研究院, 杭州 310058
- 林耀奔
- 浙江大学土地与国家发展研究院, 杭州 310058
- 胡一鸣
- 浙江大学土地与国家发展研究院, 杭州 310058
- 摘要：为探究土地整治对重金属污染环境下土壤理化性质及其中微生物的影响，本文基于Illumina高通量测序技术，对比分析了工业区周边土地整治区内外的土壤微生物群落结构和多样性.结果表明：①从土壤性质来看，土地整治区重金属污染状况和土壤质量均优于非整治区.②从土壤微生物群落结构来看，水田土壤微生物群落主要的菌门为变形菌门、绿弯菌门、酸杆菌门，而土地整治可能降低了变形菌门相对丰度，提高了绿弯菌门和酸杆菌门的相对丰度，同时在属水平上也提高了鞘脂单胞菌属、地杆菌属的相对丰度.③从土壤微生物多样性来看，土地整治区中土壤微生物多样性和功能性明显得到改善，但随着重金属污染程度的升高，改善效果逐渐减弱.④Spearman相关性分析表明，Cd、Hg、Pb、pH、AP是影响微生物群落的主要环境因子，而硫杆菌属、芽孢杆菌属、鞘脂单胞菌属与多种重金属元素表现出显著的正相关关系.本研究证实了土地整治有助于改善土壤微生物多样性和功能性，并提升土壤质量.
- Abstract：In order to explore the effects of land consolidation on soil and microbes in heavy metal polluted environment, this paper comparatively analyzed the microbial diversity and community structure inside and outside the land consolidation area around the industrial area by Illumina high-throughput sequencing technology. The results showed that:① In terms of soil properties, heavy metal pollution situation and soil quality in land consolidation area were better than those in non-consolidation area. ② From the perspective of community structure, Proteobacteria, Chloroflexi and Acidobacteria were the dominant phyla in paddy soil; land consolidation may reduce the relative abundance of Proteobacteria, increase the relative abundance of Chloroflexi and Acidobacteria, and concurrently increase the relative abundance of Sphingomonas and Geobacteria at genus level. ③From the view of microbial diversity, soil microbial diversity and function in land consolidation area were obviously improved, but the improvement was gradually weakened with the increase of heavy metal pollution. ④According to Spearman's correlation, Cd, Hg, Pb, pH and AP were main environmental factors affecting microbes, while Thiobacillus, Bacillus and Sphingomonas showed significant positive correlation with various heavy metal elements. This study confirmed that land consolidation could ameliorate microbial diversity and function, and improve soil quality.
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