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研究报告

摘要：含酚含氮废水的处理一直以来都是石油化工等行业关注重点之一.本研究采用UASB反应器，通过反硝化微生物与厌氧氨氧化微生物的耦合作用，成功实现了对甲酚、硝酸盐和氨氮的同步去除.在温度为30℃，进水pH为7.0，HRT为12 h，进水对甲酚、硝酸盐和氨氮浓度在75、78和91 mg·L^-1的条件下，3种污染物的去除率为95%、100%和73%以上，去除的硝酸盐和氨氮大部分转化为N₂，去除的对甲酚尚未矿化.当对甲酚、硝酸盐和氨氮的负荷为0.195、0.189和0.234 kg·m^-3·d^-1时，系统3种污染物的去除稳定在94%、75%和93%以上.微生物分析结果表明，门水平微生物主要有Chloroflexi、Planctomycetes、Acidobacteria、和Proteobacteria，属水平上Candidatus Kuenenia是主要的厌氧氨氧化微生物，Bacillus是主要的反硝化微生物，二者协同作用完成对甲酚、硝酸盐和氨氮的同步去除.

Abstract：p-Cresol- and ammonium- laden wastewater treatment is one of the critical concerns for petroleum and chemical industry. In this study, the simultaneous removal of p-cresol, nitrate and ammonium was achieved in a UASB reactor by inoculating denitrification and anammox bacteria. At 30℃, pH 7.0 and HRT of 12 h, influent concentrations of p-cresol, nitrate and ammonium at 75, 78 and 91 mg·L^-1, receptively, the removal efficiency reached higher than 95%, 100% and 73%. Most nitrate and ammonium removed were converted to N₂, although p-cresol was still not mineralized. When the influent loading was 0.195、0.189 and 0.234 kg·m^-3·d^-1 for p-cresol, nitrate and ammonium, respectively, the removal efficiencies maintained higher than 94%, 75% and 93%. Chloroflexi, Planctomycetes, Acidobacteria and Proteobacteria were the predominant bacteria. Candidatus Kuenenia was the main anammox bacteria at genus level and Bacillus were the main heterotrophic denitrification bacteria. The simultaneous removal of p-cresol, nitrate and ammonium was achieved with the cooperation of Candidatus Kuenenia and Bacillus.