袁鹏飞,董俊青,彭晓春,卓敏,李爽.一种新的水质遗传毒性评价方法的建立[J].环境科学学报,2020,40(1):284-289
一种新的水质遗传毒性评价方法的建立
- Establishment of a novel method for genotoxicity estimation of water
- 基金项目：广州市科技计划项目民生科技攻关专题（No.201903010086，201704020183）；广东省科技计划项目（No.2017A010105019）
- 袁鹏飞
- 华南理工大学生物科学与工程学院, 广州 510006
- 董俊青
- 华南理工大学生物科学与工程学院, 广州 510006
- 彭晓春
- 广州德隆环境检测技术有限公司, 广州 511400
- 卓敏
- 华南理工大学生物科学与工程学院, 广州 510006
- 李爽
- 华南理工大学生物科学与工程学院, 广州 510006
- 摘要：为了便于低成本、快速地检测环境中各种污染物的遗传毒性，本研究以前期构建的UV敏感菌株为监测菌，对多种已知化合物的遗传毒性进行测试.结果显示该菌株对遗传毒性化合物可快速产生响应，与化合物孵育0.5 h后裸眼即可观察到菌液浊度发生变化，且受试菌菌液浊度呈现剂量依赖的特点.剂量效应曲线计算表明，该菌株对两性霉素B、氯化苄、克菌丹和萘啶酮酸的最低检出浓度分别为0.32 mmol·L-1、0.11 mmol·L-1、0.07 μmol·L-1和2.14 μmol·L-1，均低于欧盟标准遗传毒性测试方法——SOS/umu方法.进一步建立了Cr（VI）的剂量-效应标准曲线，为评价复杂样品的遗传毒性强度提供了一个可能的选择.利用该方法对3个环境水样进行了测试，其中一个水样在浓缩200倍后可诱使测试菌发生SOS响应，其Cr（VI）等效剂量浓度为0.08 μmol·L-1 Cr（VI）.上述结果表明，该方法具有应用于环境样品遗传毒性定性与半定量初筛的潜力.
- Abstract：In order to quickly detect the genotoxicity of various pollutants in the environment, a UV-sensitive strain constructed in previous study was used to monitor the genotoxicity of various known compounds. The strain showed quick responses when incubated with the tested compound. The biosensor responded only after 0.5 h contact with mutagens and the changes in cell culture turbidity could be easily differentiated with naked eyes from the control sample. Especially, cell densities presented obvious dose-dependent characteristics. The values of limit of detection (LOD) for amphotericin B, benzyl chloride, captan and nalidixic acid were calculated as 0.32 mmol·L-1, 0.11 mmol·L-1, 0.07 μmol·L-1 and 2.14 μmol·L-1, respectively, which were much lower than that in EU standards (SOS/umu method). Using Cr (VI) as the reference compound, a potential genotoxicity estimation method was established for water samples. Three water samples were tested by this method and the obvious SOS response was observed in one sample. The toxic equivalent concentration was estimated as 0.08 μmol·L-1 Cr (VI) when the water sample was concentrated by 200 times. This provided an optional detection method in water sample genotoxicity detection.
