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研究论文

谢孟星,钱新,刘彤,李小龙,范一凡,陆昊,袁俣.基于微生物完整性指数的河流健康评价 ——以无锡市为例[J].环境科学学报,2020,40(3):1112-1120

摘要：生物完整性指数（Index of biotic integrity，IBI）被广泛运用于河流的健康评价.然而，随着人类活动对河流影响的增加，传统指示生物多样性的损失降低了评价的准确性.在高度受影响的水体环境中，微生物群落被证明是更敏感的生态健康指标.本研究采用Illumina高通量测序技术，于2018年冬季和2019年春季对无锡市境内8条河流的微生物群落组成进行了分析，从群落组成/相对丰度、丰富度/多样性、敏感性/耐受性3个角度确定了候选指标.并对传统的核心指标筛选方法进行了改进，通过差异性分析、箱线图检验、Speraman相关性分析，建立了冬、春两季的微生物生物完整性指标框架.评价结果表明，M-IBI能有效区分不同程度的受损位点，且与水质状况和物理生境存在显著的相关性.总体而言，M-IBI为城市河流生态状况提供了一种新的评价工具.

Abstract：Index of biotic integrity (IBI) has been widely applied in health assessment of rivers. However, with the increasing anthropic impact on urban rivers, the biodiversity losses of local macroorganism species reduced the accuracy of bioassessment. Microbial communities thus have been proved to be more sensitive indicators of ecological health in highly impacted aquatic environment. This study applied illumina high-throughput DNA sequencing technique to analyze the microbial community diversity and composition of the selected 8 urban rivers in Wuxi city in winter 2018 and spring 2019. A dataset of candidate metrics was first compiled, and classified into three categories:composition/abundance, richness/diversity, and sensitivity/tolerance. This study improved the traditional candidate indicator screening method, conducted difference analysis, boxplot test, and Speraman correlation, and ultimately developed the microbial biological integrity index (M-IBI) framework for habitat evaluation in winter and spring. The results showed that M-IBI was able to effectively discriminate different impaired site groups, and had a good correlation with the index of water quality and the physical habitat index, indicating that the M-IBI is a reliable and novel assessment tool for assessing the ecological health status of urban rivers.

Key words：urban rivers index of biotic integrity(IBI) ecosystem health assessment microbiome index of biotic integrity (M-IBI)