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研究论文

王慎,张思思,许尤,官卓宇,杨正健,刘德富,马骏.水库沉积物贫营养型好氧反硝化菌(Pseudomonas sp.)分离及其对微污染水体脱氮效率分析[J].环境科学学报,2020,40(3):915-921

Isolation of aerobic denitrifying bacteria(Pseudomonas sp.) from reservoir sediments and analysis of its denitrification efficiency in micro-polluted water

刘德富
1. 湖北工业大学土木建筑与环境学院, 河湖生态修复与藻类利用湖北省重点实验室, 武汉 430068;2. 三峡大学, 三峡库区生态环境教育部工程研究中心, 宜昌 443002

摘要：为探究深水水库沉积物微生物功能特征及利用价值，于2019年在实验室对小湾水库表层沉积物微生物进行了驯化分离，并分析了其中一株细菌的脱氮效率.结果表明，分离出的细菌XW731经鉴定属于假单胞菌属（Pseudomonas sp.），是一种贫营养型好氧反硝化菌；在分别以NH₄⁺-N、NO₃^--N和NO₂^--N为唯一氮源时，该菌对NH₄⁺-N、NO₃^--N和NO₂^--N去除率分别为33.6%、68.5%和9.1%；以NH₄⁺-N和NO₃^--N为氮源时，对NH₄⁺-N和NO₃^--N去除率分别为66.4%、89.6%，同步硝化反硝化能力更强.将该菌投加到两种城市微污染水体后测试表明，该菌对城市河道水体的NH₄⁺-N和NO₃^--N去除率分别为38.3%和42.4%，对城市降雨水体的NH₄⁺-N和NO₃^--N去除率分别为22.2%和7.7%.

Abstract：In order to study the characteristics and application of microorganisms in sediments of deep-water reservoir, the microorganisms in surface sediments of the Xiaowan reservoir were cultivated and isolated in the laboratory, and the denitrification efficiency of one isolate was analyzed. The results show that the isolate, XW731, is a type of oligotrophic aerobic denitrifying bacteria, which belongs to Pseudomonas. When NH₄⁺-N, NO₃^--N and NO₂^--N was the sole nitrogen source, respectively, the removal rates of NH₄⁺-N, NO₃^--N and NO₂^--N were 33.6%, 68.5% and 9.1%. when NH₄⁺-N, NO₃^--N were nitrogen sources, the removal rates of NH₄⁺-N, NO₃^--N were 66.4%, 89.6%, meanwhile, simultaneous nitrification and denitrification ability were stronger. Furthermore, the bacteria were added to two kinds of micro-polluted water, XW731 had certain denitrification effect and the removal rates of NH₄⁺-N, NO₃^--N were 38.3%, 42.4% respectively in urban river water, and 22.2%, 7.7% respectively in urban rainfall water.

Key words：aerobic denitrifier heterotrophic nitrification nitrogen removal characteristics